Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.26 (invertase)
 4,927 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Using a new selection protocol we have identified and preliminarily characterized three new loci (ADR7, ADR8 and ADR9) which affect ADH2 (alcohol dehydrogenase isozyme II) expression. Mutants were selected which activate ADH2 expression in the presence of an over-expressed, normally inactive ADR1 allele. The mutants had very similar phenotypes with the exception that one was temperature sensitive for growth. In the absence of any ADR1 allele, the mutants allowed ADH2 to partially escape glucose repression. However, unlike wildtype strains deleted for ADR1, the mutants were able to efficiently derepress ADH2. The mutations allowed a small escape from glucose repression for secreted invertase, but had no effect on the glucose repression of isocitrate lyase or malate dehydrogenase. The mutations were shown to be nonallelic to a wide variety of previously characterized mutations, including mutations that affect other glucose-repressed enzymes.
 ...
PMID:Identification and characterization of three genes that affect expression of ADH2 in Saccharomyces cerevisiae. 142 33

By fusing the upstream activation sequence (UAS) of ADH2 gene to the 5' end of a series of deletions in the upstream region of SUC2 gene, 4 expression plasmids YFD110 delta 1, YFD110 delta 9, YFD110 delta 17 and YFD110 delta 11 were constructed. They contained different hybrid promoters for transcription of SUC2 gene. After transforming them together with two control plasmids YFD26 delta 1, YFD25 into yeast. S. cerevisiae Y33 respectively, the transformants were grown in the repression or derepression media and the invertase produced by each transformants were analyzed by colormetry and gel electrophoresis. The results were as follow is: (1) UASSUC2 and UASADH2 in the hybrid plasmid YFD110 delta 1 worked synergically under derepression condition. Under repression condition, Y33/YFD110 delta 1 produced very low level of glycosylation invertase. (2) Compared with three different derepression media, the medium containing low concentration glucose gave higher derepression efficiency of glycosylated invertase than the media containing glycerol and ethanol.
 ...
PMID:[Construction of yeast ADH2-SUC2 hybrid promoter and its regulation in gene expression]. 957 60