Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.26 (invertase)
 4,927 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A simple and reliable test for the diagnosis of hyposucrasia is required, since this may be an unsuspected cause of long-standing gastrointestinal disorder. Furthermore little has been done to define the epidemiology of this condition, possibly because of the limitations of multiple blood-sampling. Breath hydrogen (H2) production after lactose ingestion is a reliable test for hypolactasia, and has now been measured after sucrose ingestion in eleven patients with various gastrointestinal symptoms. Six who had normal sucrase activity on jejunal biopsy produced no H2 after taking 50 g of sucrose. No H2 was produced in three patients with borderline hyposucrasia, either after 50 g sucrose or when retested using 100 g sucrose (two patients). However, the two patients with low jejunal sucrase activity showed rises of breath H2, after only 25 g glucose. Breath H2 measurement is a simple, accurate, and non-invasive test for diagnosing gastrointestinal symptoms due to hyposucrasia.
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PMID:Breath hydrogen in hyposucrasia. 5 37

The sucrose breath test (SBT) is a simple noninvasive technique used currently to determine intestinal absorptive function in humans and rodents. However, to date, the test has not been adapted for use in swine. During weaning, intestinal sucrase activity in piglets temporarily declines in response to stressors and is commonly used as a marker of the intestinal response to weaning. Here we assessed the sucrose dose needed for using the SBT in piglets. Six randomly allocated piglets were orogastrically gavaged with (13)C-labeled sucrose at a dose of 2 g/kg; breath samples were collected for measurement of (13)CO2 on days 0 (approximately 17 h after weaning), 5, and 10 after weaning. The resultant SBT value (cumulative dose at 90 min) was decreased by 46% on day 5 after weaning relative to baseline levels, consistent with temporal changes in gastrointestinal sucrase activity associated with weaning. We conclude that a sucrose dose of 2 g/kg is satisfactory to conduct SBT studies in piglets. With further development, the SBT may provide a new tool to noninvasively monitor digestive function in weaned piglets, to assess the effects of nutritional strategies on intestinal health, and as an indicator of gut integrity and function in swine models of human gastrointestinal disease.
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PMID:Using the noninvasive (13)C-sucrose breath test to measure intestinal sucrase activity in swine. 2356 84