Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.26 (
invertase
)
4,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The Candida utilis yeast, which is cultivated in liquid media enriched with saccharose, synthesizes the well-known
invertase
of 300 kDa
(
EC 3.2.1.26
). This enzyme is present both intracellularly in the periplasmic space and extracellularly in the culture broth. However, it was determined that the same C. utilis strain cultured in certain conditions is simultaneously capable of producing another, still unknown form of
invertase
with a molecular mass of 60 kDa. The presence of the latter enzymatic form was detected in cells as well as in the liquid culture medium. Both
invertase
forms were purified using a three-step process (ion-exchange chromatography, affinity chromatography, and preparative column electrophoresis) and named, due to their different migration ratio in polyacrylamide gel electrophoresis, F-form (Fast; 60 kDa) and S-form (Slow; 300 kDa). The F-form of
invertase
was found to be nonglycosylated as opposed to the well-known S-form of
invertase
from the same source. The physicochemical properties of the F-form of
invertase
(isoelectric point, substrate specificity, pH, and temperature optima) were determined and compared with those of the S-form of the enzyme.
...
PMID:The novel non-glycosylated invertase from Candida utilis (the properties and the conditions of production and purification). 1182 7
