Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.23 (
beta-galactosidase
)
14,648
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
WARP
is a recently described member of the von Willebrand factor A domain superfamily of extracellular matrix proteins, and is encoded by the Vwa1 gene. We have previously shown that
WARP
is a multimeric component of the chondrocyte pericellular matrix in articular cartilage and intervertebral disc, where it interacts with the basement membrane heparan sulfate proteoglycan perlecan. However, the tissue-specific expression of
WARP
in non-cartilaginous tissues and its localization in the extracellular matrix of other perlecan-containing tissues have not been analyzed in detail. To visualize
WARP
-expressing cells, we generated a reporter gene knock-in mouse by targeted replacement of the Vwa1 gene with
beta-galactosidase
. Analysis of reporter gene expression and
WARP
protein localization by immunostaining demonstrates that
WARP
is a component of a limited number of distinct basement membranes.
WARP
is expressed in the vasculature of neural tissues and in basement membrane structures of the peripheral nervous system. Furthermore,
WARP
is also expressed in the apical ectodermal ridge of developing limb buds, and in skeletal and cardiac muscle. These findings are the first evidence for
WARP
expression in non-cartilaginous tissues, and the identification of
WARP
as a component of a limited range of specialized basement membranes provides further evidence for the heterogeneous composition of basement membranes between different tissues.
...
PMID:The extracellular matrix protein WARP is a novel component of a distinct subset of basement membranes. 1831 16
