Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.23 (beta-galactosidase)
 14,648 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Microarray analysis revealed that the expression of ferric reductase (FRP1) can be regulated by the Riml01 protein. In order to find new transcriptional regulatory element in the promoter of FRP1, we analyzed the 1000 bp sequence upstream of ATG to find 2 potential Riml01p binding sites. We generated site-specific mutations in each of the two sites and fused these mutated promoters to LacZ. Then the promoter-LacZ fusion construct was recombinant into wild type and riml01-/- strains for beta-galactosidase assay. The results revealed that the FRP1 was up-regulated in alkaline pH and this was caused by iron starvation. The -650 site, not the -160 site, had an important role in FRP1 Riml01p-dependent expression. We conclude that Riml01p may interact with the -650 binding site of the promoter to regulate the FRP1 expression.
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PMID:[Regulating promoter element of iron-dependent gene FRP1 in Candida albicans by site-directed mutation]. 1899 34

The opportunistic pathogen Candida albicans develops different systems to acquire essential nutrients such as iron. The Rim101 pathway controls gene expression to adapt to different environments through transcription factor Rim101p. Ferric reductase genes are important for iron acquisition, but further work should be carried out to show about how ferric reductase genes are regulated. In this study we demonstrate that ferric reductase FRP1 expression is upregulated by alkaline pH and iron-limited conditions. Moreover, promoter deletion identified that the upregulated elements lie in -822 to 437 and 263 to -124 and the downregulated elements in -437 to -263 using a beta-galactosidase reporter system. Using electrophoretic mobility shift assay we also found that Rim101p can bind directly with two putative 5'-CCAAGAA-3' sites at the -157 and -629 sites. Interestingly, site-specific mutations show that only the -629 site, not the -157 site, plays an important role in FRP1 expression under iron-limited conditions.
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PMID:Candida albicans ferric reductase FRP1 is regulated by direct interaction with Rim101p transcription factor. 1907 41