Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.23 (beta-galactosidase)
 14,648 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A 6.4-kb sequence upstream of the mouse blue visual pigment gene has been assayed in transgenic mice for the ability to direct cell-type-specific expression of a linked beta-galactosidase (lacZ) reporter. The construct is expressed specifically in cone photoreceptors in three independent lines. Transgene expression is found in the developing retina on the first postnatal day, increases rapidly in subsequent days, and persists through adulthood. A gradient of transgene expression is observed across the retina, with the transgene-expressing cones found almost exclusively in the lower retina and rarely in the upper retina, a pattern that parallels the distribution of blue cones in the mouse retina. Double-labeling with anti-cone pigment antibodies shows that transgene expression is confined to blue cones. These results imply that all of the sequence elements necessary for the control of blue cone-specific expression are encoded within the 6.4-kb DNA fragment tested.
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PMID:A sequence upstream of the mouse blue visual pigment gene directs blue cone-specific transgene expression in mouse retinas. 791 27

Sequences 5' of the human blue visual pigment gene have been assayed in transgenic mice for their ability to direct cell-type-specific expression of linked beta-galactosidase (lacZ) and placental alkaline phosphatase (P ALP1) reporters. Constructs containing either 5.4 kilobases (kb) or 0.47 kb of 5' flanking DNA direct expression exclusively to the retina. Within the retina, transgene expression is confined to blue cones and cone bipolar cells, as determined, respectively, by double labeling with anti-cone pigment antibodies and by morphologic analyses. These results imply that blue cones and cone bipolar cells have partially overlapping transcriptional specificities.
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PMID:Blue cones and cone bipolar cells share transcriptional specificity as determined by expression of human blue visual pigment-derived transgenes. 820 64