Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.23 (
beta-galactosidase
)
14,648
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A CArG box motif [CC(A+T-rich)6GG] is one of the DNA elements required for muscle-specific gene transcription. Nuclear factors in mouse C2 myogenic cells strongly bind to the CArG box in the first intron of the gene (Sm alpha-A) encoding human smooth muscle alpha-actin. To clone cDNAs of the CArG box-binding factor (CBF), lambda gt11 cDNA expression libraries from C2 cells were screened for in situ DNA binding specific for this CArG box sequence. The 1.6-kb cDNA (
CBF-A
) encoding 285 amino acids (aa) was obtained, and a
beta-galactosidase
fusion protein, bacterially produced from the cDNA, bound to DNA fragments containing several CArG boxes. When the expression level of
CBF-A
in C2 cells increased by transfection of
CBF-A
expression plasmids, Sm alpha-A transcription was repressed. The deduced aa sequence of
CBF-A
is similar to some single-stranded (ss) nucleic acid-binding proteins. The fusion protein could bind to ssDNA, whereas CBF in C2 cell nuclear extracts could not. From these results,
CBF-A
is a novel CArG box-, ssDNA- and RNA-binding protein, as well as a repressive transcriptional factor.
...
PMID:A protein binding to CArG box motifs and to single-stranded DNA functions as a transcriptional repressor. 139 4
