Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.23 (
beta-galactosidase
)
14,648
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The gene that encodes the general transcription factor known as
BTF3
was disrupted in mouse embryonic stem cells in a random mutagenesis screen for developmental genes with the ROSA beta-geo (Friedrich and Soriano, 1991) retroviral gene trap vector. The
BTF3
mutation was transmitted through the germline of chimaeric mice. While the endogenous
BTF3
gene is ubiquitously expressed, the expression pattern of the
beta-galactosidase
reporter gene present in the gene trap vector in
BTF3
heterozygotes was restricted. Mice homozygous for the mutant allele died soon after implantation, around embryonic day 6.5. Thus,
BTF3
is essential for postimplantation development. The isolation of the
BTF3
sequences in this ROSA beta-geo insertion was facilitated by a relatively simple single lacZ primer reverse transcription PCR strategy.
...
PMID:An insertional mutation in the BTF3 transcription factor gene leads to an early postimplantation lethality in mice. 765 15
