Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.23 (beta-galactosidase)
 14,648 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Long-chain acyl-CoA synthetases (LACSs) activate fatty acids for further metabolism and are encoded by a multi-gene family in Arabidopsis. AtLACS6 possesses a type 2 (PTS2) peroxisomal targeting sequence, whilst AtLACS7 has both a type 1 and type 2 peroxisomal targeting sequence. AtLACS7 was used as bait in a yeast two-hybrid screen. Multiple clones of the PTS1 receptor PEX5 were isolated. Quantitative beta-galactosidase assay indicated that full-length PEX5 interacts with AtLACS7 with higher affinity than the TPR domains alone. The interaction between PEX5 and AtLACS7 was confirmed by co-immunoprecipitation and shown to be specific for the PTS1, therefore the AtLACS7 PTS1 is accessible to bind PEX5 in the full-length AtLACS7 protein. The expression profile of AtLACS6, AtLACS7, AtPEX5, and AtPEX7 revealed that AtLACS6 and 7 have distinct patterns of expression and we speculate that the possession of two targeting signals may be advantageous for the import of AtLACS7 when receptors may be limiting.
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PMID:AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5. 1625 65

MYB transcription factor is one of the largest families in plants, which plays an important role in regulating plant development and physiological metabolism. In this study, the expression and function of the new MYB transcription factor gene GmMYBJ6 (GenBank No. DQ902863), isolated from soybean (Glycine max L.), were characterized. The expression pattern of GmMYBJ6 in different organs was examined using Northern blotting analysis. The expression of GmMYBJ6 was detected only in the leaves. The transcriptional activation ability of GmMYBJ6 protein was confirmed by the yeast assay system and the activity of beta-galactosidase was 28.48 U/mL. The green fluorescent protein expression vector p163-GFP-GmMYBJ6 was constructed and transformed into the epidermal cells of onion via particle bombardmental method. The results of instantaneous expression showed that GmMYBJ6 proteins were localized in cell nucleus. Semi-quantitative RT-PCR analysis indicated that GmMYBJ6 improved the expression of certain flavonoid biosynthetic genes, such as PAL (Phenylalanine ammonia lyase), C4H (cinnamate-4-hydroxylase), 4CL (4-coumaroyl-CoA ligase), CHS (Chalcone synthase), CHI (Chalcone isomerase), F3H (Flavanone 3-hydroxylase), and FLS (Flavonol synthase), resulting an increase of the total flavonoid levels in positive tobacco transformants. Additionally, the increasing expression of GmMYBJ6 in soybean cultivar Zhongdou 27, induced by UV-B radiation, drought, and high-salt treatment, indicated that GmMYBJ6 was associated with response to abiotic stresses.
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PMID:[Expressing and functional analysis of GmMYBJ6 from soybean]. 1958 66


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