Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.23 (beta-galactosidase)
 14,648 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

[3H]Fucose, intravenously injected into androgen-treated mice, was incorporated at high rates into the immunopurified kidney lysosomal enzymes beta-glucuronidase and beta-galactosidase. Initially, label was incorporated into high molecular weight proenzyme forms. Processing of fucose-labeled proenzyme forms to lower molecular weight mature forms was very rapid, being detectable at 30 min, and complete by 90 min, compared with the several hours required for processing of lysosomal enzymes labeled with amino acids. This result is consistent with addition of fucose residues within the Golgi apparatus just before transfer of lysosomal proenzyme forms to the lysosome where maturation is thought to occur. The combination of the high rates of incorporation of [3H]fucose and the known metabolic stability of this precursor sugar suggests that the mouse kidney system is advantageous for studies of the synthesis, processing, and degradation of fucose-containing complex oligosaccharides of lysosomal enzymes and, by extension, of other kidney glycoproteins.
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PMID:Synthesis and processing of lysosomal enzymes of mouse kidney after treatment with [3H]fucose. 313 58

The infective third-stage larvae (L3s) of Strongyloides ratti, a parasitic nematode in rodents, showed two types of chemokinesis on a gradient of sodium chloride (NaCl) in an in vitro agarose tracking assay. The types were a consistent directional avoidance behavior under unfavorable environmental conditions and a reduced avoidance behavior under favorable conditions. We examined the effects of treatments with glycolytic enzymes and lectins by analyzing the avoidance behavior. L-Fucose dehydrogenase, hyaluronidase, beta-glucosidase, alpha-mannosidase, beta-galactosidase, concanavalin A, wheat germ agglutinin and soybean agglutinin exhibited inhibitory or enhancive effects on chemokinesis. We also confirmed the sites of the amphids of L3s aside from the mouth at the anterior end by scanning electron microscopy, and that concanavalin A-binding sites existed in the vicinity of the amphids using lectin-histochemistry. The carbohydrate moieties in the amphids of S. ratti L3s may play an important role as chemosensors in perceiving environmental cues.
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PMID:Strongyloides ratti: chemokinesis of glycolytic enzyme- and lectin-treated third-stage infective larvae in vitro. 1586 77