Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.23 (
beta-galactosidase
)
14,648
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Two cDNA clones (lambda
GC-1
and lambda GC-2) for human beta-glucocerebrosidase [EC 3.2.1.45] have been isolated from a human hepatoma library in lambda gt11 by immunological screening using monospecific polyclonal antibody for beta-glucocerebrosidase. Restriction endonuclease mapping indicates that these clones are probably identical in size, each with a 1900 bp insert. The 50 kDa size of the insert-encoded polypeptide produced by these clones in fusion with
beta-galactosidase
of lambda gt11 in E. coli BNN103 is consistent with the size of the nascent form of beta-glucocerebrosidase. These fusion proteins are shown by Western blotting to react with antibody to beta-glucocerebrosidase. Amino acid sequence deduced from the nucleotide sequence of the insert ir pGC-1 is identical to known amino acid sequence of beta-glucocerebrosidase, and thus, confirms that the clones are specific for beta-glucocerebrosidase.
...
PMID:Isolation of cDNA clones for human beta-glucocerebrosidase using the lambda gt11 expression system. 609 33
