Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.23 (
beta-galactosidase
)
14,648
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A headspace gas chromatographic method for the determination of
beta-galactosidase
(E.C. 3.2.1.23) activity is described. The method, in which
2,2,2-trichloroethyl
beta-D-galactopyranoside (beta-TCG) is used as substrate, involves determination of the liberated 2,2,2-trichloroethanol by gas chromatography with electron capture detection. The preparation of beta-TCG and of
2,2,2-trichloroethyl
alpha-D-galactopyranoside is described. A Km = 0.80 mM was found for the enzymatic hydrolysis of beta-TCG employing
beta-galactosidase
from Escherichia coli. The assay has been applied to the quantitative determination of E. coli bacteria.
...
PMID:Headspace gas chromatographic determination of beta-galactosidase activity using electron-capture detection. 392 58
