Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.23 (beta-galactosidase)
 14,648 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

[14C]Lactose electroinjected into isolated rat hepatocytes is normally autophagocytosed, transferred to lysosomes and degraded by lysosomal beta-galactosidase, but at high concentrations of asparagine the transfer is inhibited and lactose accumulates in prelysosomal autophagic/endocytic vacuoles (amphisomes). The accumulation can be prevented by addition of yeast beta-galactosidase, which is transferred to the lactose-containing vacuoles by endocytosis. Propylamine, a weak base capable of neutralizing acidic vacuoles, protects autophagocytosed lactose against both endogenous and exogenous beta-galactosidase, suggesting that amphisomes, like lysosomes, have an acidic internal environment.
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PMID:Evidence for acidity of prelysosomal autophagic/endocytic vacuoles (amphisomes). 847 Oct 30