Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.23 (beta-galactosidase)
 14,648 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A macromolecular antigen, ornithine delta-aminotransferase [EC 2.6.1.13] from rat liver (OAT) was assayed by the sandwich procedure using rabbit (anti-OAT) Fab'-beta-D-galactosidase complex and rabbit (anti-OAT) IgG-coupled glass rods as a solid phase. The Fab' fragments of the rabbit (anti-OAT) IgG were conjugated with beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N, N'-o-phenylenedimaleimide. The rabbit (anti-OAT) IgG was coupled to the aminoalkylsilyl glass rods using glutaraldehyde. The rabbit (anti-OAT) IgG-coupled glass rods were incubated with OAT and then with the rabbit (anti-OAT) Fab'-beta-D-galactosidase complex. The amount of OAT was determined from the activity of beta-D-galactosidase bound to the glass rods. A minimum of 0.03 fmoles of OAT could be determined by this method and use of the glass rods gave greater reproducibility, and was more sensitive and simpler than use of Sepharose 4B.
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PMID:Enzyme-linked sandwich immunoassay of ornithine delta-aminotransferase from rat liver using antibody-coupled glass rods as solid phase. 79 9

The article summarises data on the activity of tubular enzymes having marked organ-specific characteristics related to kidneys. They are N-acetyl-beta-D-glucosaminidase (NAG), its thermostable isoenzyme NAG B and beta-galactosidase localised in lysosomes. L-canavinine, ornithine aminotransferase having mitochondrial localization have also been discussed in the article. The authors have dealt also with the activity of lipoperaxidation processes having been assessed by an ammount of malonic dialdehyde in blood serum, erythrocytes and urine of 51 patients with progressive diabetes mellitus at the late stages of diabetic nephropathy.
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PMID:[Activity of tubular enzymes and lipoperoxidation processes in patients with progressive diabethic nephropathy]. 1668 87