Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.23 (
beta-galactosidase
)
14,648
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
An experimental study was undertaken to quantify the effects of infection cell density, medium condition, and surface aeration on recombinant protein yields in insect cells. In the absence of surface aeration and fresh medium, insect cells generated higher product yields (on a per cell basis) when infected with recombinant baculovirus at low cell densities,
LCD
(3 x 10(5)-4 x 10(5) cells/mL), than at high cell densities, HCD (>0.9 x 10(6) cells/mL), for two distinct baculovirus types. Surface aeration of a HCD culture infected in spent medium improved
beta-galactosidase
yields 5-fold over the nonaerated case. Surface aeration and medium replenishment improved
beta-galactosidase
yields of a HCD culture by 20-fold (compared to a 1.6-fold improvement for a
LCD
culture), resulting in cultures with productivties that were independent of the cell density at infection.
...
PMID:Quantification of cell culture factors affecting recombinant protein yields in baculovirus-infected insect cells. 1860 Sep 90
