Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.23 (
beta-galactosidase
)
14,648
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
MBP-1
acts as a general transcriptional repressor. Overexpression of
MBP-1
induces cell death in a number of cancer cells and regresses tumor growth. However, the function of endogenous
MBP-1
in normal cell growth regulation remains unknown. To unravel the role of endogenous
MBP-1
, we knocked down
MBP-1
expression in primary human foreskin fibroblasts (HFF) by RNA interference. Knockdown of
MBP-1
in HFF (HFF-MBPsi-4) resulted in an induction of premature senescence, displayed flattened cell morphology, and increased senescence-associated
beta-galactosidase
activity. FACS analysis of HFF-MBPsi-4 revealed accumulation of a high number of cells in the G1-phase. A significant upregulation of cyclin D1 and reduction of cyclin A was detected in HFF-MBPsi-4 as compared to control HFF. Senescent fibroblasts exhibited enhanced expression of phosphorylated and acetylated p53, and cyclin-dependent kinase inhibitor, p21. Further analysis suggested that promyolocytic leukemia protein (PML) bodies are dramatically increased in HFF-MBPsi-4. Together, these results demonstrated that knockdown of endogenous
MBP-1
is involved in cellular senescence of HFF through p53-p21 pathway.
...
PMID:Knockdown of MBP-1 in human foreskin fibroblasts induces p53-p21 dependent senescence. 1885 84
