Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.23 (
beta-galactosidase
)
14,648
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Transient transfection of COS-1 cells with an expression vector for
NIPP-1
, a nuclear subunit of protein phosphatase-1, did not result in an overexpression of
NIPP-1
protein, although the levels of mRNA encoding
NIPP-1
increased dramatically. Moreover, high concentrations of
NIPP-1
mRNA inhibited the translation in reticulocyte lysates of various unrelated mRNAs. This inhibition of translation was caused by the
NIPP-1
messenger and not by the translation product, since mutation of the start codon abolished
NIPP-1
protein production, but had no influence on the translational inhibition. Analysis of deletion mutants showed that the inhibition was mediated by a 0.5-kb fragment in the 5'-end of the
NIPP-1
mRNA. This region, when inserted in the 5'-untranslated region of the
beta-galactosidase
messenger, inhibited the translation of
beta-galactosidase
mRNA in COS-1 cells. A predicted highly stable secondary structure deltaG = -239.5 kJ/mol) is present between residues 300 and 500 of
NIPP-1
mRNA. The possible importance of this structure in the translational inhibition is discussed.
...
PMID:Inhibition of translation by mRNA encoding NIPP-1, a nuclear inhibitor of protein phosphatase-1. 924 54
