Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.23 (
beta-galactosidase
)
14,648
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A "cleavage cassette" specifying a decapeptide human immunodeficiency virus (HIV) protease cleavage site was introduced into six different locations of
beta-galactosidase
(
beta-D-galactoside galactohydrolase
,
EC 3.2.1.23
) in Escherichia coli. Four of these constructs retained
beta-galactosidase
activity despite the insertion of the cleavage cassette. Of these four constructs, one was cleaved by HIV protease, resulting in the inactivation of
beta-galactosidase
both in vivo and in vitro. This cleavage was inhibited by pepstatin A, a known inhibitor of HIV protease. Thus,
beta-galactosidase
has been converted into an easily assayed substrate for HIV protease. An analogous construct of
beta-galactosidase
containing a
polio
protease cleavage site was cleaved likewise by
polio
protease, suggesting that this system may be generic for monitoring cleavage by a variety of proteases.
...
PMID:beta-Galactosidase containing a human immunodeficiency virus protease cleavage site is cleaved and inactivated by human immunodeficiency virus protease. 212 94
