Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.21 (
beta-glucosidase
)
3,280
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Clostridium sp. and some bacterial cellulases exist as an enzyme complex with cellulolytic, and hemicellulolytic enzymes, so called "cellulosome". In this article, we report that EF-CMCase25 occurs as a complex with
beta-glucosidase
, beta-1,3 glucanase, and beta-xylosidase. The multienzyme complex had a molecular mass of 150 kDa on gel filtration under non-reducing condition. After the gel filtration, the enzyme complex was purified to homogeneous state on BN-PAGE. The SDS-PAGE demonstrated that the purified protein is a complex with at least one CMCase (25 kDa), one
beta-glucosidase
(32 kDa), and one beta-1,3 glucanase (
40 kDa)
components. The CMCase activity in the purified enzyme complex at 15 degrees C was 44% of that obtained at the optimal temperature. The optimum pH of the EF-CMCase25 in the purified enzyme complex was pH 5.0 and stable at pH 7.0-9.0.
...
PMID:A novel cold-adapted cellulase complex from Eisenia foetida: characterization of a multienzyme complex with carboxymethylcellulase, beta-glucosidase, beta-1,3 glucanase, and beta-xylosidase. 2043 57
