Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.21 (
beta-glucosidase
)
3,280
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
This paper investigates how BST204, a fermented ginseng extract, affects the expression and mechanism of
cyclooxygenase-2
(
COX-2
). BST204 was prepared by incubating crude ginseng extract with ginsenoside-
beta-glucosidase
. Unexpectedly, BST204 had no effect on the level of
COX-2
protein in unstimulated RAW 264.7 cells, and it suppressed the level of
COX-2
protein and PGE(2) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. It did not show any suppressive effect, though, on the
COX-2
mRNA level. To investigate the suppressive mechanism of
COX-2
protein, the activating phosphorylation of p70 S6 kinase and 4E-BP1, which are important for translation, were measured. The phosphorylation of p70 S6 kinase, not 4E-BP1, was increased by LPS in a time-dependent manner, and was inhibited by BST204 in a dose-dependent manner. The expression of
COX-2
protein, however, was partially suppressed by rapamycin, an upstream inhibitor of p70 S6 kinase. Therefore, this paper suggests that the suppression of
COX-2
protein by BST204 was partially correlated with the inhibition of p70 S6 kinase activation.
...
PMID:Effect of a fermented ginseng extract, BST204, on the expression of cyclooxygenase-2 in murine macrophages. 1577 28
