Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.21 (beta-glucosidase)
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Orange seedlings were cultured in aqueous solutions of 3-tert-butyl-5-chloro-6-methyluracil (terbacil and terbacil-2-14C). Radioactivity was distributed throughout the plant with the largest amount in the roots and the smallest amount in the leaves. Terbacil was metabolized to form 3-tert-butyl-5-chloro-6-hydroxymethyl uracil, which was conjugated to form a beta-glucoside as the conjugate was hydrolyzed by beta-glucosidase. Identification of the metabolite was made by infrared and mass spectrometry after isolation and purification by column and thin layer chromatography. An additional unidentified water-soluble material was accumulated in the plant. 5-chloro-uracil was not detected as a metabolite of terbacil.
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PMID:Metabolism of terbacil in orange seedlings. 119 Aug 39