Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.21 (beta-glucosidase)
 3,280 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The RapID-ANA System (Innovative Diagnostics Systems, Inc., Atlanta, Ga.) was used to test 102 strains of 14 species of phenotypically similar bile-inhibited Bacteroides from humans. Bacteroides oris, Bacteroides veroralis, Bacteroides buccalis, Bacteroides melaninogenicus, Bacteroides loescheii, and Bacteroides denticola had very similar enzyme activity profiles. Clear differentiation of these six species by the RapID-ANA System was not possible, but tests for arginine aminopeptidase and beta-glucosidase were helpful. Bacteroides oralis, Bacteroides intermedius, Bacteroides corporis, Bacteroides disiens, Bacteroides bivius, Bacteroides gingivalis, Bacteroides asaccharolyticus, and Bacteroides buccae each had unique enzyme activity profiles. No consistent differences in enzyme activities were found between the two DNA homology groups within Bacteroides melaninogenicus, Bacteroides loescheii, or Bacteroides intermedius. Tests for glycine aminopeptidase, alpha-galactosidase, arginine aminopeptidase, alpha-fucosidase, N-acetylglucosaminidase, reduction of triphenyltetrazolium, and production of indole were helpful in the differentiation of the species studied.
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PMID:Use of the RapID-ANA System to screen for enzyme activities that differ among species of bile-inhibited Bacteroides. 287 Oct 42

Frequent incidence of Serpulina strains showing all cultural and biochemical characteristics of Serpulina (S.) hyodysenteriae except of being indole negative, and alpha-galactosidase positive isolates showing strong haemolysis on Columbia agar with 5% sheep blood and trypticase soy agar with 5% ox blood, respectively, was the cause to evaluate common biochemical and cultural methods in Serpulina routine diagnostics. To this purpose ten type and reference strains as well as 47 field strains were examined for their ability to produce indole, haemolysis, hippurate cleavage, alpha-galactosidase, alpha- and beta-glucosidase activity. Two four-hour identification-systems were used, RapID ANA II and Rosco diagnostic tablets. The ability to produce indole was determined by different methods. All investigations were carried out at least two times. For the investigation of haemolytic patterns trypticase soy agar with 10% ox blood proved to be most effective. Results received using this agar could always be confirmed by the ring phenomenon. Determining the ability to produce indole by adding p-dimethylaminocinnamaldehyde to bacterial growth collected on a cotton swab was confirmed to be more sensitive than other methods. Both four-hour-systems were shown to be useful in Serpulina diagnostics, though in the RapID ANA II only four of 18 available reactions could be used and the hippurate cleavage reaction has to be carried out additionally. Using cultural and biochemical methods, it was possible to assign the type and reference strains to the correct species, as well as 46 of 47 field isolates could be identified including all five known intestinal Serpulina species from swine. 27 strains were determined as S. hyodysenteriae, nine of these isolates atypically being indole negative. In contrast one canine S. pilosicoli strain was atypical showing indole production. Therefore incidence of indole negative variants of S. hyodysenteriae as well as indole positive S. pilosicoli isolates must be taken into consideration.
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PMID:[The diagnosis of swine dysentery and spirochaete diarrhea. 1. Cultural-biochemical differentiation of intestinal Serpulina in routine diagnosis]. 1037 1