Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.21 (beta-glucosidase)
 3,280 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Incorporation into the wall of Candida albicans Ssr1, a GPI-dependent protein, was investigated by construction of different truncated genes for which the three potential omega sites (S199, S215 and G216) and the corresponding omega+1 and omega+2 were eliminated or modified. Cells of the C. albicans ssr1Delta mutant were transformed with pADH-pl harboring the truncated versions of CaSSR1, pADH-DeltaCaSSR1t(217-234) (lacking a C-terminal hydrophobic stretch of 18 aa including the putative omega+2 and omega+1, omega+2 of S215 and G216) or pADH-DeltaCaSSR1t(199-201) (lacking three serine residues), and their walls were analyzed for the protein. Results suggested that the three serine residues are essential for incorporation of CaSsr1 into the wall beta-glucan. This interpretation was confirmed when the truncated protein CaSsr1pt(199-201) was found in the spent medium. The transcription profile of the 6039 genes in C. albicans ssr1Delta showed that seven genes are upregulated (1.4-fold), including SRP54 (a signal recognition particle subunit), IPF29 (a zinc finger protein) and PTR3 (a transcriptional regulator), whereas 27 genes are downregulated (0.7-fold), including IPF6318 (a beta-glucosidase) and SOU1 (a sorbitol utilization protein). Additional genes showed a reduced increase, or decreased expression, suggesting that some current orphan genes may have unknown cell wall functions. In addition, a compensatory mechanism would appear to occur, as a substantial increase in the amount of beta-1,3-glucan (2.34-fold) was detected in the cell wall of the mutant cells.
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PMID:Anchorage of Candida albicans Ssr1 to the cell wall, and transcript profiling of the null mutant. 1602 27

The INDETERMINATE1 (ID1) transcription factor is a key regulator of the transition to flowering in maize. ID1 is expressed in immature leaves where it controls the production or transmission of leaf-derived florigenic signals. Loss-of-function id1 mutants make many more leaves than normal plants and produce aberrant flowers; however, they exhibit no obvious developmental defects in early growth stages. A maize oligonucleotide microarray was used to assess the molecular differences between immature leaves of wild-type and id1 mutant plants prior to the floral transition. This analysis revealed 55 genes with a significant 2-fold difference in expression; 22 are down-regulated and 33 are up-regulated in id1 mutants. Most prominent is a novel family of three beta-glucosidase genes that are most closely related to sorghum dhurrinases. These genes, termed Zmdhr1, Zmdhr2, and Zmdhr3, are undetectable in immature leaves of id1 mutants and are expressed exclusively in normal immature leaves in a pattern identical to the ID1 gene. Other down-regulated genes include a group of four zinc finger protein-encoding genes that are unrelated to ID1. A significant number of genes up-regulated in id1 mutant immature leaves have potential roles in photosynthesis and carbon fixation, substantiating a possible connection between floral induction and assimilate partitioning. Finally, expression of these genes was compared in florally induced versus uninduced teosinte, a photoperiod-sensitive progenitor of day-neutral maize. Only a few genes showed expression differences, suggesting that ID1 acts in a novel autonomous floral induction pathway that is distinct from the photoperiod induction pathway.
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PMID:Expression differences between normal and indeterminate1 maize suggest downstream targets of ID1, a floral transition regulator in maize. 1792 72