Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.21 (beta-glucosidase)
 3,280 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The filamentous fungus Penicillium italicum when grown in a synthetic medium, produced and secreted 1,6-beta-glucanase into the culture medium. This enzyme has been partially purified by gel filtration. After this step the active fractions were free of 1,3-beta-glucanase, alpha-amylase and beta-glucosidase activities. Only four proteins, one associated with the enzyme, were found by polyacrylamide gel electrophoresis under non denaturing conditions. The enzyme behaves as an acidic protein (pI 4.65) with an optimum pH of 5 and an endohydrolytic mode of action. The activity was also found associated with the mycelium. Its synthesis was repressed by glucose or growth-promoting sugars. Derepression in low glucose containing medium required protein synthesis. 8-Hydroxyquinoline, an RNA synthesis inhibitor, added during the derepression period did permit some increase in the specific activity but prevented it when added at the beginning of that period.
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PMID:Characterization and synthesis regulation of Penicillium italicum 1,6-beta-glucanase. 4 70

8-Hydroxyquinoline-beta-D-glucoside has been shown to be a useful alternative substrate for the differentiation of members of the family Enterobacteriaceae based on the presence or absence of beta-glucosidase. In an identification protocol based on multipoint-inoculated plates 8-hydroxyquinoline-beta-D-glucoside when incorporated into an agar based medium supplemented with a ferric salt produced intense black pigmentation localised solely in the immediate vicinity of beta-glucosidase positive organisms. In contrast, using conventional aesculin plates, positive organisms produced a brown ferric-aesculetin complex, the diffusibility of which limited interpretation especially where large numbers of colonies were involved. Almost complete agreement was found when 1964 routine 'coliform' organisms were tested by both methods. The method described allows use of a 36 place 'minipin' inoculator without any danger of 'nearest neighbour' interference and constitutes a cost-effective method suitable for either manual or automatic reading.
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PMID:Detection of specific bacterial enzymes by high contrast metal chelate formation. Part I. 8-Hydroxyquinoline-beta-D-glucoside, an alternative to aesculin in the differentiation of members of the family Enterobacteriaceae. 312 22