Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.21 (beta-glucosidase)
 3,280 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The authors present the results of study or fegularities attending the changes in the activity of free, total and bound fractions of the lysosomal enzymes--beta-glucosidase and beta-galactosidase in the thymus and the spleen of rabbits under conditions of DOCA administration. The activity of the enzymes was studied 30 min, 1, 4, 12, 24 and 48 hours after a single injection of the hormone. DOCA administration caused biphasic changes in the activity of both glycosidases. A marked increase in the activity of all the enzyme fractions during the first experimental hours was later replaced by their fall. An increase in the activity of glycosidases at the early periods of DOCA administration pointed to the intensification of the enzymatic synthesis, and also could be associated with the spicific induction of the enzymatic activity. The activity of beta-glucosidase and of beta-galactosidase directly depended on DOCA dose. Effects similar to the experiments in vivo were obtained in vitro. The activity of hyaluronidase under the effect of Dca decreased considerably in the thymus and the spleen, particularly at the early experimental periods, pointing to reduction of tissue permeability of the lymphoid organs.
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PMID:[The effect of desoxycorticosterone acetate on the activity of the lysosomal enzymes of lymphoid organs]. 113 80

Male Wistar rats received a low-protein (4.5% protein) diet during 30 days, and T-2-toxin was administered to them through a gastric tube, in a dose of 0.54 mg/kg, during 15 days. The results obtained showed activation of hepatic lysosomal enzymes (sulfatase A and B aryl and beta-glucosidase) and alkaline phosphatase, and to an essential suppression of enzymatic activity of peroxisomes - catalase, glycolate oxidase and D-amino acid oxidase. A sharp aggravation of the intoxication symptoms and pronounced intensification of changes in enzymatic activity in the liver, spleen, thymus and blood serum were recorded in the animals given T-2 toxin simultaneously with the low-protein diet.
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PMID:[Enzyme parameters in the evaluation of the combined effects of protein deficiency and T-2 mycotoxin]. 289 95

Dynamics of 5 marker enzymes activity from subcellular particles--succinate dehydrogenase, beta-glucosidase, arylsulphatases A and B, acid RNAase and glucose-6-phosphatase--were studied in liver, spleen, thymus and blood serum of rats after single intragastric administration of T-2 toxin at a dose of 3.8 mg/kg (LD50). The acute intoxication was accompanied by an early (within 1-3 hrs) and significant reduction of total proteins in the tissues studied. Distinct tissue- and organelle-tropic effects of T-2 toxin were found: the toxin induced a gradual decrease in activity of the enzymes studied in liver tissue, while selective activation of lysosomal hydrolases was observed in spleen and thymus tissues. The selective effect of T-2 toxin on spleen and thymus lysosomal hydrolases appear to be involved in the mechanism of action of the toxin.
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PMID:[Effect of T-2 toxin on the activity of organelle-specific enzymes of various rat organs]. 631 90

The time-course of the ultrastructural changes and activities of 6 marker enzymes of subcellular particles (succinate dehydrogenase, beta-glucosidase, beta-N-acetylglucosaminidase, acid RNAse, glucose-6-phosphatase and 5'-nucleotidase) has been studied in the liver, spleen and thymus in rats administered T-2 toxin (mycotoxin produced by some Fusarium species). A pronounced difference in the effect of T-2 toxin on the organs has been found. In the liver, the toxin induced a destruction of rough endoplasmic reticulum membranes, reduced ribosome number and progressively decreased activities of most enzymes. In the spleen, early and significant ultrastructural disturbances of all the cell membrane components and simultaneous lysosomal activation were observed. The changes in the thymus were characterized by a fast development of cell hydratation, organelle swelling and necrosis of some thymocytes with parallel increase in repair processes, infiltration by phagocytes and a selective activation of lysosomal hydrolases in the end of experimental time (72 h.). The results obtained emphasize an importance of cellular and subcellular membrane alterations in the mechanism of T-2 toxin action.
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PMID:[Effect of T-2 toxin on organ ultrastructure and organelle-specific enzyme activity in rats]. 665 69