Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.20 (
alpha-glucosidase
)
4,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In this study, we investigated structural changes in
alpha-glucosidase
during urea denaturation. Alpha-glucosidase was inactivated by urea in a dose-dependent manner. The inactivation was a first-order reaction with a monophase process. Urea inhibited
alpha-glucosidase
in a mixed-type reaction. We found that an increase in the hydrophobic surface of this enzyme induced by urea resulted in aggregation caused by unstable folding intermediates. We also simulated the docking between
alpha-glucosidase
and urea. The docking simulation suggested that several residues, namely THR9,
TRP14
, LYS15, THR287, ALA289, ASP338, SER339, and TRP340, interact with urea. Our study provides insights into the
alpha-glucosidase
unfolding pathway and 3D structure of
alpha-glucosidase
.
...
PMID:Alpha-glucosidase folding during urea denaturation: enzyme kinetics and computational prediction. 1941 60
