Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.20 (alpha-glucosidase)
 4,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In Saccharomyces cerevisiae, genes encoding maltose permeases and maltases are located in the telomeric regions of different chromosomes. The COMPASS methylation complex, which methylates lysine 4 on histone H3, controls the silencing of telomeric regions. Yeast strains deleted for SWD1, SWD3, SDC1, SET1, BRE2, or SPP1, encoding components of the COMPASS complex, fermented a medium containing 22% maltose with noticeably higher attenuation than did the wild type, resulting in production of up to 29% more ethanol. The least effective strain was spp1. Absence of COMPASS components had no effect on the fermentation of media with 20% glucose, 20% sucrose, or 16% maltose. Deletion of SWD3 resulted in larger amounts of MAL12 transcript, encoding maltase, at the late stages of fermentation of 22% maltose. A similar effect on maltase activity and maltose uptake capability was seen. The lysine 4 residue of histone H3 was trimethylated in wild-type cells at the late stages, while only small amounts of the dimethylated form were detected. Trimethylation and dimethylation of this residue were not detected in strains deleted for SWD1, SWD3, SET1, BRE2, or SDC1. Trimethylated lysine 4 was apparent only at the early stages (48 and 96 h) of fermentation in an spp1 strain. This work indicates that the COMPASS complex represses the expression of maltose utilization genes during the late stages of fermentation of a high concentration of maltose.
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PMID:Fermentation of high concentrations of maltose by Saccharomyces cerevisiae is limited by the COMPASS methylation complex. 1698 Apr 27

Carbon reserves in rice straw before flowering contribute greatly to grain filling. Moderate soil drying imposed at the post-anthesis stage significantly promotes carbon reserve remobilization in straws of rice, but the regulation of this process at the proteomic and transcriptomic level remains poorly understood. In this study, we applied moderate soil drying (MD) to rice at the post-anthesis stage, which was followed by dynamic proteomic and transcriptomic studies using SWATH-MS and RNA-seq analysis. MD treatment upregulated the proteins alpha-glucosidase, beta-glucosidase and starch phosphorylase, which are responsible for starch degradation. Furthermore, MD treatment enhanced the expression of proteins involved in the sucrose synthesis pathway, including SPS8 and SPP1. In addition, various monosaccharide transporters (MSTs) and sucrose transporter 2 (SUT2), which are pivotal in carbon reserve remobilization, were also upregulated in straw by MD treatment. Differentially expressed transcription factors, including GRAS, TCP, trihelix, TALE, C3H, and NF-YC, were predicted to interact with other proteins to mediate carbon reserve remobilization in response to MD treatment. Further correlation analysis revealed that the abundances of most of the differentially expressed proteins were not correlated with the corresponding transcript levels, indicating that the carbon reserve remobilization process was probably regulated by posttranscriptional modification. Our results provide insights into the molecular mechanisms underlying the regulation of carbon reserve remobilization from straw to grain in rice under MD conditions.
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PMID:Regulation of gene expression involved in the remobilization of rice straw carbon reserves results from moderate soil drying during grain filling. 3162 Nov 35