Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.20 (
alpha-glucosidase
)
4,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Giant-cell formation induced by macrophage fusion factor (MFF) was not altered after pretreatment of macrophages with trypsin, chymotrypsin, pronase, neuraminidase, phospholipase C, or
phospholipase D
. Pretreatment of macrophages with either alpha-mannosidase or
alpha-glucosidase
completely inhibited giant-cell development, without altering macrophage viability. No alteration of giant-cell formation was observed when 0.1 M of L-fucose, N-acetyl-glucosamine, D-arabinose, D-xylose, melibiose, D-glucose, D-galactose, alpha-lactose, sucrose, D-fructose, or maltose was present during incubation of macrophages with MFF. Giant-cell formation was abolished when 0.1 M alpha-D-mannose was present during macrophage incubation with MFF. These results suggest that the protein moiety of MFF recognizes a specific receptor site on the macrophage membrane, one that is different from those described for other lymphokines and contains alpha-mannose.
...
PMID:Chemical nature of the interaction between macrophage fusion factor and macrophage membranes. 635 71
A total of 34
Corynebacterium
sp. strains were isolated from caseous lymph node abscesses of wild boar and roe deer in different regions of Germany. They showed slow growth on Columbia sheep blood agar and sparse growth on Hoyle's tellurite agar. Cellular fatty acid analysis allocated them in the
C. diphtheriae
group of genus
Corynebacterium
. MALDI-TOF MS using specific database extensions and
rpoB
sequencing resulted in classification as
C. ulcerans
. Their quinone system is similar to
C. ulcerans
, with major menaquinone MK-8(H2). Their complex polar lipid profile includes major lipids phosphatidylinositol, phosphatidylinositol-mannoside, diphosphatidylglycerol, but also unidentified glycolipids, distinguishing them clearly from
C. ulcerans
. They ferment glucose, ribose and maltose (like
C. ulcerans
), but do not utilise d-xylose, mannitol, lactose, sucrose and glycogen (like
C. pseudotuberculosis
). They showed activity of catalase, urease and
phospholipase D
, but variable results for alkaline phosphatase and
alpha-glucosidase
. All were non-toxigenic,
tox
gene bearing and susceptible to clindamycin, penicillin and erythromycin. In 16SrRNA gene and RpoB protein phylogenies the strains formed distinct brancheswith
C. ulcerans
as nearest relative.Whole genome sequencing revealed the unique sequence type 578, a distinctbranch in pangenomic core genome MLST, average nucleotide identities <91%, enhancedgenome sizes (2.55 Mbp) and G/C content (54.4 mol%) compared to related species.These results suggest that the strains represent a novel species, for which wepropose the name
Corynebactriumsilvaticum
sp. nov., based on their first isolation from forest-dwellinggame animals. The type strain isKL0182
T
(= CVUAS 4292
T
= DSM 109166
T
= LMG 31313
T
= CIP 111 672
T
).
...
PMID:
Corynebacterium silvaticum
sp. nov., a unique group of NTTB corynebacteria in wild boar and roe deer. 3236 99
