Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.2.1.20 (alpha-glucosidase)
4,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The ability of the markers alpha-glucosidase, carnitine, and glycerylphosphorylcholine to indicate epididymal obstruction was studied. alpha-Glucosidase was found to be the best discriminant, with 93.4% correct classifications. The possible interrelationship between alpha-glucosidase activity, sperm ATP, and epididymal function was then studied. alpha-Glucosidase correlated positively with the percentage of motile sperm (p = 0.0212) and with the percentage of sperm with good forward progression (p = 0.0374), but correlated negatively with the sperm ATP (p < 0.0500). It was concluded that lower ATP and higher alpha-Glucosidase may be markers of efficient epididymal function. The possible epididymal origin of detached ciliary tufts (DCTs) was studied by determining ATP and alpha-glucosidase activity in patients with DCTs. The mean ATP in patients with DCTs was significantly higher (p = 0.009), but the alpha-glucosidase activity significantly lower (p = 0.0412) than in the control group. These results seem to support the epididymal origin of DCTs.
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PMID:Epididymal markers in an andrology clinic. 827 47

Regional differences in the total activities of the epididymal secretory parameter, alpha-glucosidase, were demonstrated in the 20,000 g supernatants of human epididymal homogenates. A comparison of the enzymic activities in the supernatants and the washings from 12 one centimetre segments of human epididymides indicated an activity peak in segments 3-4 which appeared to be largely intracellular and which presumably reflects the acidic isoenzyme. A second peak in the caudal region, the segmental localization of which was more variable and differed in post-mortem and operation specimens, appeared to be primarily intraluminal. The activities of ornithine decarboxylase (ODC), the rate-limiting enzyme in the polyamine pathway, also exhibited regional differences with higher activities in relatively short segments in both caput and caudal portions. Corresponding alterations were also found in the tissue concentrations of the enzyme products, spermidine and spermine. The increased intracellular activities of ODC and alpha-glucosidase in the distal caput segments presumably reflect the transition of epithelium from the efferent to the epididymal ducts.
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PMID:Segmental distribution of alpha-glucosidase, ornithine decarboxylase and polyamines in the human epididymis. 850 29

Neutral alpha-glucosidase activity was measured in seminal plasma of 30 patients consulting for infertility with a variety of disorders, and compared with 25 normal controls. Significantly more of the study populations (asthenozoospermia, 4/6; oligozoospermia, 3/5; azoospermia 8/15) had a neutral alpha-glucosidase activity less than 10 mIU/mL as compared with control samples (3/25). Also the mean (+/- SD) neutral alpha-glucosidase activity in patients with asthenozoospermia (11.7 +/- 9.2) oligozoospermia (11.3 +/- 7.2), and post vasectomy azoospermia (5.3 +/- 3.4) was significantly (p < .05) less than ejaculate from controls (21.2 +/- 14). A seminal alpha-glucosidase level of 10 mIU/mL appears to be a useful discriminatory marker in the diagnosis of epididymal obstruction or dysfunction.
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PMID:New discriminatory level for glucosidase activity to diagnose epididymal obstruction or dysfunction. 855 28

Antiobesity and antidiabetic actions of the alpha-glucosidase inhibitor AO-128 were examined using genetically obese-diabetic rats, Wistar fatty. Ten-week-old, male fatty rats were kept on CE-2 diet containing 10 or 25 ppm of AO-128 for 4 weeks. The average drug intake was calculated to be 0.74 or 1.78 mg/kg/day from the average food intake, respectively. The intestinal maltase and sucrase activities were decreased by AO-128 in a dose-related fashion. Food intake of fatty rats treated with AO-128 was decreased throughout the experiment. This decrease in food intake could hardly be explained only by diarrhea which occurred for the first 5 days of the administration of AO-128. AO-128 normalized hyperglycemia and markedly reduced hypertriglyceridemia and hyperinsulinemia in fatty rats. In addition, AO-128 decreased body weight gain, food efficiency, epididymal adipose tissue weight, carcass weight, and body fat deposition. These findings indicate that AO-128 may be useful for treating human obesity and diabetes.
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PMID:AO-128, alpha-glucosidase inhibitor: antiobesity and antidiabetic actions in genetically obese-diabetic rats, Wistar fatty. 869 66

Treatment of couple infertility due to male subfertility by means of intra-uterine insemination (IUI) gives better results, in terms of per cycle and total cumulative pregnancy rate, if sperm preparation is performed using a discontinuous Percoll gradient than if centrifugation-resuspension is used. Also, the minimal semen requirements for successful IUI are lower with the former technique. Optimal epididymal function, with total alpha-glucosidase activity in seminal plasma > 83 IU/mL or Schorr stain result > 60%, is associated with a high probability of success of IUI [odds ratio (OR) = 11.1 and 9.4 respectively; p < 0.01]. If semen contains > 2.3 million white blood cells per mL or more than 13 million spermatozoa/mL with grade a motility the success rate is decreased (OR = 0.25 and 0.30 respectively; p < 0.05 and p < 0.01). It is concluded that IUI is a highly successful treatment in specific cases of male subfertility, provided that the correct technique of sperm preparation is used.
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PMID:Statement on intra-uterine insemination. 871 64

In order to improve the selection of couples for intrauterine insemination (IUI) because of longstanding primary infertility of alleged male origin, we have performed a prospective study measuring conventional and advanced analysis of sperm characteristics, the hypoosmotic swelling test, the Shorr stain, the acidified aniline blue stain and alpha-glucosidase activity in seminal plasma, of 89 couples with no demonstrable abnormality of the female partner. Twenty-four couples attained spontaneous conception, 23 were successful within six cycles of IUI, and 42 remained without conception in spite of IUI during six unstimulated cycles. The proportion and concentration of spermatozoa with progressive motility was significantly lower (P < 0.01) in the successful IUI cases than in the couples attaining spontaneous conception, and the lower quartile value was lower in the former than in the latter. There were less pregnancies among IUI treated couples when sperm concentration and motility were within the range of normal fertile men, or when the concentration of white blood cells was elevated. More pregnancies occurred when markers of epididymal function, namely the result of the Shorr stain and alpha-glucosidase measurement, were normal. Total progressive motility and the result of the Shorr stain were the only independent variables selected by logistic regression to discriminate between successful and failed IUI cases. It is concluded that only a limited group of couples may benefit from IUI.
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PMID:The value of semen characteristics and tests of sperm function in selecting couples for intra-uterine insemination. 880 Nov 36

Neutral alpha-glucosidase levels as epididymal marker, fructose levels as vesicular marker, zinc, citric acid and prostate specific antigen levels as prostatic markers were measured in the seminal plasma of eight transfusion-dependent beta-thalassemic patients in order to study epididymal and sex accessory gland secretions (eighteen subjects served as controls). FSH and LH as well as total and free testosterone were detected displaying unaltered serum values. Ejaculate of patients showed normal sperm count and low sperm motility, in the meantime seminal plasma exhibited unaltered both neutral alpha-glucosidase and fructose values but low levels of zinc, citric acid and prostate specific antigen were noticed as well. These data suggest an impaired prostatic secretion in the thalassemic patients studied. A local iron toxicity on the prostatic tissue could be supported by the decrease of its specific markers observed only in the subgroup of patients with high ferritin serum levels.
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PMID:Epididymal and sex accessory gland secretions in transfusion-dependent beta-thalassemic patients: evidence of an impaired prostatic function. 922 14

The estimation of alpha-glucosidase activity in semen is widely used as a marker of epididymal function. In the present studies, glucosidase activity was evaluated in the different segments of the rat epididymis under various physiological conditions. In addition, the effect of two known male antifertility agents, gossypol and alpha-chlorohydrin, on enzyme activity was evaluated. Enzyme activity was absent from the epididymis of rats aged 10 and 20 days but became detectable at 30 days of age when the adult pattern of distribution (highest activity in the caput epididymis) was established. Enzyme activity was reduced significantly in all segments of the epididymis at 7 days after castration and a significant decrease in activity was also observed following the administration of either gossypol or alpha-chlorohydrin. These findings are consistent with a role for alpha-glucosidase in sperm maturation in the epididymis.
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PMID:alpha-Glucosidase activity in the rat epididymis under different physiological conditions. 929 19

Low alpha-glucosidase activity in seminal plasma is a marker of epididymal obstruction. The criterion standard for determining activity is the epididymal specific method, whereby the neutral iso-enzyme, specifically produced by the epididymis, is measured. A kit that determines total alpha-glucosidase activity (neutral iso-enzyme and the acid iso-enzyme originating from the prostate) has become available. The objective of the laboratory-based study was to compare alpha-glucosidase activity values measured by both the EpiScreen and the epididymal specific method, to determine if the kit may provide reliable results to substitute the neutral iso-enzyme specific method in the routine clinical setting. The neutral iso-enzyme activity according to both methods was measured in seminal plasma of 23 post-vasectomy and 24 normozoospermic patients. Significant differences (P < 0.05) were found between the activities measured according to both methods, but these differences pertained mostly to high values (> 40 mU ejaculate-1), which was not clinically significant. In conclusion, the epididymal specific method is best suited for research purposes, but the EpiScreen kit is convenient for routine use in infertility clinics.
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PMID:A comparative study of the EpiScreen kit and a conventional method for the determination of seminal alpha-glucosidase activity. 963 92

SDS-PAGE (12.5%) analysis and neutral alpha-glucosidase, fructose, and zinc level assessment were carried out in seminal plasma of 20 patients with highly viscous ejaculates and of 20 control subjects, with the aim to investigate the relations between high consistency of semen and epididymal, vesicular, and prostatic secretions. Very low sperm motility was observed in all the patients' ejaculates, both normo- and oligozoospermics. Protein patterns obtained in control and highly viscous semina showed similar protein bands, in the range of 10-100 kD. Furthermore, unaltered seminal neutral alpha-glucosidase, zinc, and fructose level were measured in the same specimens. These results indicated no impairment of epididymal, vesicular, and prostatic function in patients with hyperviscous semina, while their normal electrophoretic seminal protein profile suggested unaltered genital fluid interactions during the semen coagulation-liquefaction process.
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PMID:Unaltered protein pattern/genital tract secretion marker levels in seminal plasma of highly viscous human ejaculates. 964 58


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