Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.20 (alpha-glucosidase)
 4,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We determined the complete nucleotide sequence of the yeast MAL6R gene from the Saccharomyces carlsbergensis MAL6 locus. The MAL6R gene encodes a transacting protein required for the inducible, coordinate expression of the two divergently transcribed structural genes, MAL6T (maltose permease), and MAL6S (maltase) at this locus. The transcription initiation sites for MAL6R were determined by primer extension experiments. The MAL6R gene contains an open reading frame of 473 amino acids with a calculated Mr of 54,892. The N-terminus of the deduced protein contains an amino acid sequence isologous to a consensus sequence for cysteine-zinc associated DNA binding fingers found in other fungal DNA binding proteins. The MAL6R gene was mapped to chromosome VIII by using OFAGE (orthagonal field alternating gel electrophoresis) gels and hybridization with specific chromosome and MAL6 probes.
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PMID:Primary structure of the regulatory gene from the MAL6 locus of Saccharomyces carlsbergensis. 285 10

Maltose fermenting strains of Saccharomyces cerevisiae have one or more complex loci called MAL. Each locus comprises at least three genes: MALx1 encodes maltose permease, MALx2 encodes maltase, and MALx3 encodes an activator of MALx1 and MALx2 (x denotes one of five MAL loci, with x = 1, 2, 3, 4, or 6). The MAL43c allele is constitutive and relatively insensitive to glucose repression. To understand better this unique phenotype of MAL43c, we have isolated several MAL63c constitutive mutants from a MAL6 strain. All constitutive mutants remain glucose repressible, and all have multiple amino acid substitutions in the C-terminal region, now making this region of Mal63cp similar to that of Mal43cp. These changes have been generated by gene conversion, which transfers DNA from the telomeres of chromosome II and chromosome III or XVI to chromosome VIII (MAL6). The removal of a Mig1p binding site from the MAL63c promoter leads to a loss of glucose repression, imitating the phenotype of MAL43c. Conversely, addition of a Mig1p binding site to the promoter of MAL43c converts it to glucose sensitivity. Mig1p modulation of Mal63p and Mal43p expression therefore plays a substantial role in glucose repression of the MAL genes.
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PMID:Removal of Mig1p binding site converts a MAL63 constitutive mutant derived by interchromosomal gene conversion to glucose insensitivity. 877 May 84

The presence of any one of the five unlinked MAL loci (MAL1, MAL2, MAL3, MAL4 and MAL6) confers the ability to ferment maltose on the yeast Saccharomyces cerevisiae. Each locus is composed of three genes encoding maltose permease, alpha-glucosidase and MAL activator. Chromosomal DNA of seven representative baking strains has been separated by pulse-field gel electrophoresis and probed with three genes in MAL6 locus. The DNA bands to which all of the three MAL-derived probes simultaneously hybridized were chromosome VII carrying MAL1 in all of the strains tested, chromosome XI carrying MAL4 in six strains, chromosome III carrying MAL2 in three strains and chromosomes II and VIII carrying MAL3 and MAL6, respectively, in the one strain. The number of MAL loci in baking strains was comparable to those of brewing strains.
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PMID:Detection of maltose fermentation genes in the baking yeast strains of Saccharomyces cerevisiae. 898 1