Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.20 (
alpha-glucosidase
)
4,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Activity gel assays require a long incubation time (several hours) on renaturation of enzymatic activity after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). To reduce the incubation time, we used a novel renaturation buffer containing cyclic oligosaccharide beta-cyclodextrin (beta-CD) which is capable of capturing SDS. Yeast
alpha-glucosidase
, used as a model protein, was run on SDS-PAGE, and then the gel matrix was incubated in a variety of renaturation buffers. Compared with conventional renaturation buffers containing Triton X-100 or
isopropanol
, our novel renaturation buffer containing beta-CD can restore enzymatic activity within 10 min. Therefore, this new format represents a good alternative with reduced incubation time for activity gel assays.
...
PMID:Effect of beta-cyclodextrin on the renaturation of enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 1860 88
