Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.20 (
alpha-glucosidase
)
4,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A new mechanism-based glucosidase inactivator, conduritol
aziridine
(1,2-dideoxy-1,2-epimino-myo-inositol), has been synthesised from myo-inositol. This
aziridine
inactivates both the beta-glucosidase from Alcaligenes faecalis and the
alpha-glucosidase
from yeast according to the expected pseudo-first order kinetics. Inactivation constants measured are Ki = 3.0mM, ki = 0.077 min-1 for the beta-glucosidase, and Ki = 9.5mM, ki = 0.39 min-1 for the
alpha-glucosidase
. Evidence for irreversible inactivation is provided by the lack of reactivation upon dilution of inactivated enzyme into buffer containing substrate.
...
PMID:Conduritol aziridine: a new mechanism-based glucosidase inactivator. 267 41
