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Enzyme
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Query: EC:3.2.1.20 (
alpha-glucosidase
)
4,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Here we report the complete and finished genome sequence of Streptomyces glaucescens GLA.O (
DSM
40922), a natural producer of the
alpha-glucosidase
inhibitor acarbose, which is used in the treatment of type-2 diabetes mellitus. The genome of S. glaucescens GLA.O consists of two replicons, the chromosome with a size of 7,453,200bp and a G+C content of 73.0% as well as a plasmid named pSglau1 with a size of 170,574bp and a G+C content of 69.06%.
...
PMID:Complete genome sequence of the actinobacterium Streptomyces glaucescens GLA.O (DSM 40922) consisting of a linear chromosome and one linear plasmid. 2549 5
Azasugars, "nitrogen in the ring" analogues of monosaccharides, are known to be distributed in select plant, fungal. and bacterial species. We identify
Chitinophaga pinensis
DSM
2588 as the first bacterial source of the plant pyrrolidine azasugar 1,4-dideoxy-1,4-aminoarabinitol (DAB-1). Comparative sequence analyses identified
C. pinensis
as a putative azasugar producer, via observation of a three-gene cluster coding for putative aminotransferase, alcohol dehydrogenase, and sugar phosphatase enzymes, similar to the previously reported azasugar biosynthetic signature identified in
Bacillus amyloliquefaciens
FZB42. Multistep fractionation of
C. pinensis
culture media guided by a
maltase
inhibition assay yielded a component with a mass consistent with the structure of DAB-1. Heterologous expression of the three-gene cluster in
E. coli
, a non-azasugar producer, led to the isolation of nectrisine, a biosynthetic precursor to DAB-1, which displayed potent slow tight binding inhibition of
maltase
. Reduction of nectrisine with NaBH
4
removed the slow tight binding inhibition kinetics, and MS analysis provided evidence for the production of a compound matching that of the isolated DAB-1 from
C. pinensis
.
1
H NMR analysis of the nectrisine produced in
E. coli
after NaBD
4
reduction produced a spectrum consistent with DAB-1 deuterated at C-1, primarily at the pro-S position. These results support the idea that the azasugar three-gene cluster represents a general biosynthetic path leading to several different compounds, which may prove useful for the identification of other azasugar-producing organisms.
...
PMID:Functional Analysis of a Gene Cluster from
Chitinophaga pinensis
Involved in Biosynthesis of the Pyrrolidine Azasugar DAB-1. 3179 83
A total of 34
Corynebacterium
sp. strains were isolated from caseous lymph node abscesses of wild boar and roe deer in different regions of Germany. They showed slow growth on Columbia sheep blood agar and sparse growth on Hoyle's tellurite agar. Cellular fatty acid analysis allocated them in the
C. diphtheriae
group of genus
Corynebacterium
. MALDI-TOF MS using specific database extensions and
rpoB
sequencing resulted in classification as
C. ulcerans
. Their quinone system is similar to
C. ulcerans
, with major menaquinone MK-8(H2). Their complex polar lipid profile includes major lipids phosphatidylinositol, phosphatidylinositol-mannoside, diphosphatidylglycerol, but also unidentified glycolipids, distinguishing them clearly from
C. ulcerans
. They ferment glucose, ribose and maltose (like
C. ulcerans
), but do not utilise d-xylose, mannitol, lactose, sucrose and glycogen (like
C. pseudotuberculosis
). They showed activity of catalase, urease and phospholipase D, but variable results for alkaline phosphatase and
alpha-glucosidase
. All were non-toxigenic,
tox
gene bearing and susceptible to clindamycin, penicillin and erythromycin. In 16SrRNA gene and RpoB protein phylogenies the strains formed distinct brancheswith
C. ulcerans
as nearest relative.Whole genome sequencing revealed the unique sequence type 578, a distinctbranch in pangenomic core genome MLST, average nucleotide identities <91%, enhancedgenome sizes (2.55 Mbp) and G/C content (54.4 mol%) compared to related species.These results suggest that the strains represent a novel species, for which wepropose the name
Corynebactriumsilvaticum
sp. nov., based on their first isolation from forest-dwellinggame animals. The type strain isKL0182
T
(= CVUAS 4292
T
=
DSM
109166
T
= LMG 31313
T
= CIP 111 672
T
).
...
PMID:
Corynebacterium silvaticum
sp. nov., a unique group of NTTB corynebacteria in wild boar and roe deer. 3236 99
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