Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.20 (
alpha-glucosidase
)
4,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Nonlipophilic corynebacteria associated with clinical and subclinical mastitis in dairy cows were found to belong to four species: Corynebacterium amycolatum, Corynebacterium ulcerans, Corynebacterium
pseudotuberculosis
, and Corynebacterium minutissimum. These species may easily be confused. However, clear-cut differences between C. ulcerans and C.
pseudotuberculosis
were found in their acid production from maltotriose and ethylene glycol, susceptibility to vibriostatic agent O129, and alkaline phosphatase. Absence of growth at 20 degrees C and lack of
alpha-glucosidase
and 4MU-alpha-D-glycoside hydrolysis activity differentiated C. amycolatum from C.
pseudotuberculosis
and C. ulcerans. The mastitis C.
pseudotuberculosis
strains differed from the biovar equi and ovis reference strains and from caprine field strains in their colony morphologies and in their reduced inhibitory activity on staphylococcal beta-hemolysin. C. amycolatum was the most frequently isolated nonlipophilic corynebacterium.
...
PMID:Identification of nonlipophilic corynebacteria isolated from dairy cows with mastitis. 1007 8
A total of 34
Corynebacterium
sp. strains were isolated from caseous lymph node abscesses of wild boar and roe deer in different regions of Germany. They showed slow growth on Columbia sheep blood agar and sparse growth on Hoyle's tellurite agar. Cellular fatty acid analysis allocated them in the
C. diphtheriae
group of genus
Corynebacterium
. MALDI-TOF MS using specific database extensions and
rpoB
sequencing resulted in classification as
C. ulcerans
. Their quinone system is similar to
C. ulcerans
, with major menaquinone MK-8(H2). Their complex polar lipid profile includes major lipids phosphatidylinositol, phosphatidylinositol-mannoside, diphosphatidylglycerol, but also unidentified glycolipids, distinguishing them clearly from
C. ulcerans
. They ferment glucose, ribose and maltose (like
C. ulcerans
), but do not utilise d-xylose, mannitol, lactose, sucrose and glycogen (like
C.
pseudotuberculosis
). They showed activity of catalase, urease and phospholipase D, but variable results for alkaline phosphatase and
alpha-glucosidase
. All were non-toxigenic,
tox
gene bearing and susceptible to clindamycin, penicillin and erythromycin. In 16SrRNA gene and RpoB protein phylogenies the strains formed distinct brancheswith
C. ulcerans
as nearest relative.Whole genome sequencing revealed the unique sequence type 578, a distinctbranch in pangenomic core genome MLST, average nucleotide identities <91%, enhancedgenome sizes (2.55 Mbp) and G/C content (54.4 mol%) compared to related species.These results suggest that the strains represent a novel species, for which wepropose the name
Corynebactriumsilvaticum
sp. nov., based on their first isolation from forest-dwellinggame animals. The type strain isKL0182
T
(= CVUAS 4292
T
= DSM 109166
T
= LMG 31313
T
= CIP 111 672
T
).
...
PMID:
Corynebacterium silvaticum
sp. nov., a unique group of NTTB corynebacteria in wild boar and roe deer. 3236 99
Periplasmic oligopeptide binding protein (OppA) is part of a multimeric cytoplasmic membrane protein complex, whose function is known as peptide transporters found in Gram-negative bacteria. A chaperone-like activity has been found for the OppA from Yersinia
pseudotuberculosis
, using biochemical experiments. Through computational analysis, we selected two amino acid residues (R41 and D42) that probably are involved in the chaperone-like activity. Our results to corroborate how OppA assists refolding and renaturation of lactate dehydrogenase and
alpha-glucosidase
denatured enzymes.
...
PMID:Identification of residues for chaperone-like activity of OppA protein in Yersinia pseudotuberculosis. 3282 76
