Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.20 (alpha-glucosidase)
 4,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

beta-Hexosaminidase (EC 3.2.1.20; Hex) activity and isoenzyme characteristics were analyzed in human normal and leukemic leukocytes. Unseparated CLL and CML cells had a specific activity that was lower, whereas ALL and AML blasts had a higher specific activity than normal lymphocytes and granulocytes. CLL B-cells had a lower specific activity compared with that in normal non-T-lymphocytes; CLL T-cells and normal T-cells had similar activity. Isoenzyme separation was performed by chromatofocusing on PBE-94 coupled with an automated enzyme assay. When using a single linear pH elution gradient, normal leukocytes and all leukemia cells contained two forms of isoenzyme (B and A). When a double pH elution gradient was performed, an extra distinct form of Hex (I) was recorded. Hex I was present in small amounts in normal granulocytes and PHA-stimulated normal lymphocytes; isoenzyme I was found in high amounts in all leukemias tested. The activity ratios I/B and I/A, as well as the I isoenzyme profile, may facilitate differentiation between normal and leukemic cells and between lymphoblastic and myeloblastic leukemias.
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PMID:Alteration of beta-hexosaminidase activity and isoenzymes in human leukemic cells. 294 28

The activity of five acid glycosidases was determined in lymphocytes from normal animals and animals with chronic lymphocytic leukemia. It was shown that alpha-D-mannosidase activity in leukemic lymphocytes was 5 times lower (p less than less than 0.001) and alpha-D-glucosidase activity was 2 times lower (p less than 0.01) than in normal controls. The progress of the disease and the increase in leukocyte count were accompanied by the decrease in alpha-D-mannosidase activity. No differences have been found in alpha-D-mannosidase properties (thermostability, Km values, ZnSl2 activation) in normal and leukemic lymphocytes.
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PMID:[Acid glycosidases of normal cattle lymphocytes and in chronic lympholeukemia]. 407 71

Leukocytes were obtained from 14 healthy subjects, one patient with the infantile form, two patients with the adult variant of acid maltase deficiency, two patients with chronic myelocytic leukemia, two patients with acute myeloid leukemia, and two patients with chronic lymphotic leukemia. In addition, lymphocytes were prepared from three normal subjects, and five established lymphoid lines were used. Cells were extracted either with Triton 0, 2%, or with water followed by 0.2% Triton. alpha-Glucosidase activity was measured in water homogenates, water extracts after centrifugation, and Triton extracts, with or without antisera directed against acid maltase (EC 3.2.1.3) and renal maltase (EC 3.2.1.20). The percentage of acid and renal maltases was then calculated in each soluble fraction. Normal whole leukocytes (mostly granulocytes) contain both acid and "renal" maltases, whereas normal lymphocytes contain very little or no "renal maltase." This isozyme is present in chronic myelocytic leukemia, but is absent in acute myeloid and chronic lymphocytic leukemia as well as in established lymphoid lines. Acid maltase is almost completely extracted with water, whereas renal maltase is extracted only with Triton. From the results, it appears that for the diagnosis of alpha glucosidase deficiency, cells should be extracted in water and centrifuged before determination. Lymphocytes, which are devoid of renal maltase, are a better diagnostic material than are granulocytes.
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PMID:White blood cells and the diagnosis of alpha-glucosidase deficiency. 676 91

Eleven lysosomal enzyme activities were tested in lymphocytes from normal individuals and patients with chronic lymphocytic leukemia. The activities of all enzymes, except acid phosphatase, were significantly lower in the leukemic lymphocytes (p less than 0.001). In addition the activities were tested in purified T- and non-T lymphocytes and in monocytes. For most enzymes the activities are similar in the three cell types, except for alpha-D-galactosidase, arylsulphatase B and alpha-D-glucosidase, which are lower in T lymphocytes, and N-acetyl-beta-D-glucosaminidase which is lower in non-T cells. In T and B lymphoblastic cell lines the activities are within the range for leukemic lymphocytes. No differences were found between the T and the B cell lines.
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PMID:Lysosomal enzymes in normal and leukemic B lymphocytes. 697 60