Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.17 (
lysozyme
)
21,489
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Two scaffold/matrix attachment regions (S/MARs) from Chinese hamster ovary (CHO) cell chromosomes were identified computationally and cloned as the domain boundaries regions. As partition chromosomes in independently regulated units to increase the exogenous gene expression, these two S/MARs (MAR1 and
MAR6
) flanked expression vectors were respectively transfected into CHO cells. The results showed that MAR1 and
MAR6
were much more potent to increase the stable transfection efficiency than the chicken
lysozyme
gene 5' S/MARs, which had been experimentally identified to increasing the expression of heterologous gene. Moreover,
MAR6
is more active in mediating high and consistent gene expression in CHO cells than the chicken
lysozyme
gene 5' S/MARs.
...
PMID:Scaffold/matrix attachment regions from CHO cell chromosome enhanced the stable transfection efficiency and the expression of transgene in CHO cells. 2439 63
