Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.17 (
lysozyme
)
21,489
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Lab-on-a-chip technology offers an ideal platform for low-cost, reliable, and easy-to-use diagnostics of key biomarkers needed for early screening of diseases and other health concerns. In this work, a graphene field-effect transistor (GFET) functionalized with target-binding aptamers is used as a biosensor for the detection of
thrombin
protein biomarker. Furthermore, this GFET is integrated with a microfluidic device for enhanced sensing performances in terms of detection limit, sensitivity, and continuous monitoring. Under this platform, a picomolar limit of detection was achieved for measuring
thrombin
; in our experiment measured as low as 2.6 pM. FTIR, Raman and UV-Vis spectroscopy measurements were performed to confirm the device functionalization steps. Based on the concentration-dependent calibration curve, a dissociation constant of K
D
= 375.8 pM was obtained. Continuous real-time measurements were also conducted under a constant gate voltage (V
GS
) to observe the transient response of the sensor when analyte was introduced to the device. The target selectivity of the sensor platform was evaluated and confirmed by challenging the GFET biosensor with various concentrations of
lysozyme
protein. The results suggest that this device technology has the potential to be used as a general diagnostic platform for measuring clinically relevant biomarkers for point-of-care applications.
...
PMID:An integrated microfluidic platform for selective and real-time detection of thrombin biomarkers using a graphene FET. 3240 Aug 15
Designing the catalytic interface that preferentially attracts reactants is highly desirable for amplifying chemiluminescence (CL) emission. Herein, to boost the generation of reactive oxygen species (ROS) from dissolved O
2
molecule, flower-like cobalt hydroxide (f-Co(OH)
2
) based catalytic interface with hierarchical and porous architecture were in situ created in the coexistence of BSA and Co
2+
. Benefiting from the oxidase-like catalysis capability and the unique microstructure of f-Co(OH)
2
, ROS was efficiently produced. Meanwhile, the capping ligands of BSA endowed the interface with the capability of enriching functionality through the interaction between BSA and luminol. 100-fold CL enhancement was achieved using the as-prepared catalytic interface compared with the classical luminol-Co
2+
or luminol-BSA system. Moreover, the proposed catalytic amplification mechanism could be extended to the different proteins such as
lysozyme
, protamine,
thrombin
, papain. Based on the quenching effect on CL, a sensitive sensing platform was constructed for the determination of ascorbic acid with satisfied results. Our finding provided a novel "all-in-one" route to design the catalytic interface for amplifying CL emission.
...
PMID:In Situ Formed Catalytic Interface for Boosting Chemiluminescence. 3254 51
In this article, we report a novel dual on/off
thrombin
fluorescence aptasensor by combining the energy driven target induced strand displacement reaction and a non-enzyme catalyst recycling DNA machine. Firstly, the specific binding of an aptamer strand and
thrombin
induce the release of a catalyst which was used as a DNA machine trigger. Subsequently, the catalyst as the trigger initiated the DNA machine through nucleic acid hybridization and branch migration of the DNA machine, resulting in the DNA substrate melting and re-hybridization. In such a working model, the DNA machine achieved cooperative control of the circular strand displacement reaction, realizing catalyst recycling and dual-amplification. The fluorescence signal change of FAM and ROX accumulation had a good linear relationship with the
thrombin
concentration in the range of 1 fM to 1 nM. On account of catalyst recycling and dual recognition, the detection limit for
thrombin
was determined to be as low as 0.45 fM (S/N = 3).This biosensor also showed good selectivity for
thrombin
without being affected by some other proteins, such as PSA,
lysozyme
etc. Moreover, this assay can be applied to the detection of
thrombin
in diluted human serum.
...
PMID:An off/on thrombin activated energy driven molecular machine for sensitive detection of human thrombin
via
non-enzymatic catalyst recycling amplification. 3282 Feb 97
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