Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mice were injected 3 times a day for 12 days with 8 micrograms/kg of somatostatin 14 which caused a hypoplasia of parietal and goblet cells, a hypotrophy and hypofunctionality of pancreatic acinar cells with a decrease in lipase and chymotrypsin activities, a decrease in the secretory fuction of the Brunner gland and in the number of dark granules of G cells. Neither villous and microvillous areas nor brush border hydrolase activities were affected. The number of peptic cells and Paneth cells increase as the level of pepsin and lysozyme. Mice were injected 4 times per hour with 2 micrograms/kg of somatostatin. 2 h after the first injection of somatostatin and 90 min after a single injection of tritiated thymidine, fundic, antral, jejunal and ileal labelling indexes strongly decrease (maximal effect in ileum). The inhibitory effect of somatostatin on the digestive epithelial cell proliferation compared to its long-term action only directed on specific cell types evokes probable compensatory mechanisms induced to maintain the equilibrium of the digestive epithelia.
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PMID:Long-term effect of somatostatin 14 on mouse stomach, antrum, intestine and exocrine pancreas. 285 47

The glomerular filtration rate (creatinine clearance), glomerular permeability (qualitative and quantitative proteinuria), tubular reabsorption (k-lambda chains of immunoglobulins and lysozyme) and indexes of tubular cell lysis (alpha-glucosidase and gamma-glutamyltranspeptidase) were measured in the urine of 10 patients with moderate, uncomplicated essential hypertension during placebo therapy and after captopril given at increasing doses of 25, 50, 100 and 200 mg twice daily, the first three doses being given for 3 days and the last one for 4 weeks in all patients and for an additional 6 months in 5 patients. During placebo therapy, proteinuria was absent in eight patients and detectable (glomerular and selective) in two; selective proteinuria appeared in two and a decrease in selectivity was observed in two patients with previous proteinuria after 4 weeks of captopril therapy. No proteinuria was detectable in the five patients followed up to 6 months, not even in the one in whom a decrease in glomerular selectivity had occurred after 4 weeks. The glomerular filtration rate was unchanged as were lysozyme and gamma-glutamyltranspeptidase values, while light chains were always undetectable. Alpha-glucosidase showed some increase; however, increments were transient and always much lower than those observed with known tubular toxic drugs. These data show that under our experimental conditions captopril caused no evident changes in glomerular and tubular function.
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PMID:Effect of captopril on renal function in patients with essential hypertension. 704 2

Methods of making molecularly ordered protein films are reviewed with special reference to the recently developed technique of protein multilayer assembly by alternated adsorption of opposite-charge polyions. This method has been applied for linear and branched polyions, DNA, polynucleotides, proteins, viruses and clay nanoplates. This provides good prospects for biomolecular architecture. Quartz crystal microbalance, X-ray and neutron reflectivity, scanning electron microscopy, atomic force microscopy and UV-absorbance data are used to analyze the film structure. Multilayer buildup by alternation of polyions and 16 different charged proteins is discussed. In most cases, enzymes in the films retained their activity. Protein/ceramic nanoplates consisting of alternated montmorillonite clay and glucose oxidase layers electrostatically linked by polycations were also assembled. Protein layers can be arranged according to specific biological activity. Consecutive enzymic reactions were performed in anisotropic protein layers prepared with precise control of distances between the active layers (1-50 nm). Film superlattices containing ordered layers of more than one protein were constructed using myoglobin, lysozyme, peroxidase, glucoamylase, glucose oxidase and catalase. Glucoamylase, glucose oxidase/peroxidase catalyze the starch-glucose-H2020 reaction. The reaction products and nonreacting starch were separated by filtration when the substrate solution passed through the multienzyme films assembled on a filter. Formation of alternate outermost layers (of opposite charge or opposite specificity) at every adsorption cycle is the key point of the layer-by-layer assembly. Multilayers were obtained by alternated adsorption of concanavalin A and glycogen (or streptavidin and biotinylated polylysine) were designed using their biospecific interaction. Protein films are of extreme interest as novel biologically active materials.
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PMID:Protein architecture: assembly of ordered films by means of alternated adsorption of oppositely charged macromolecules. 946 49

Adult Anopheles darlingi salivary glands are paired organs located on either side of the esophagus. The male glands consist of a single small lobe. The female gland is composed of two lateral lobes, with distinct proximal and distal portions, and a medial lobe. The lobes are acinar structures, organized as a unicellular epithelium that surrounds a salivary canal. The general cellular architecture is similar among the lobes, with secretory material appearing as large masses that push the cellular structures to the periphery of the organ. Cells of the proximal-lateral lobes show asynchronous cycles of secretory activity and contain secretory masses with finely filamentous aspect. In the distal-lateral lobes, cells display synchronous cycles of activity, and have a dense secretory product with mottled pattern. Cells of the medial lobe have secretory masses uniformly stained and highly electrondense. Biochemical analysis of the adult female salivary glands revealed apyrase, alpha-glucosidase and lysozyme activities. Alpha-glucosidase and lysozyme activities are detected mostly in the proximal lobes while apyrase is mainly accumulated in the distal lobes. This differential distribution of the analyzed enzymes reflects a specialization of different regions for sugar and blood feeding. Thus, the morphological differences observed in the lobes correlate with functional ones.
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PMID:Morphological and biochemical analyses of the salivary glands of the malaria vector, Anopheles darlingi. 1048 Dec 98