Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.17 (
lysozyme
)
21,489
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Lactoferrin and
lysozyme
are important antimicrobial compounds of airway surface liquid, derived predominantly from serous cells of submucosal glands but also from surface epithelium. Here we compared release of these compounds from the following human cell cultures: primary cultures of tracheal epithelium (
HTE
), Calu-3 cells (a lung adenocarcinoma cell line frequently used as a model of serous gland cells), 16HBE14o- cells (an SV40 transformed line from airway surface epithelium), T84 cells (a colon carcinoma cell line), and human foreskin fibroblasts (HFF). For
lysozyme
, baseline secretory rates were in the order Calu-3 > 16HBE14o- >
HTE
T84 > HFF = 0; for lactoferrin, the only cell type showing measurable release was
HTE
; for mucus,
HTE
> Calu-3 > 16HBE14o- T84 > HFF = 0. A wide variety of neurohumoral agents and inflammatory stimuli was without effect on lactoferrin and
lysozyme
release from
HTE
or Calu-3 cells, although forskolin did stimulate secretion of water and
lysozyme
from Calu-3 cells. However, the concentration of
lysozyme
in the forskolin-induced secretions was much less than in airway gland secretions. Thus our data cast doubt on the utility of Calu-3 cells as a model of airway serous gland cells but do suggest that
HTE
could prove highly suitable for studies of mucin synthesis and release.
...
PMID:Secretion of lactoferrin and lysozyme by cultures of human airway epithelium. 1500 37
