Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The rate constants of efficient exchange interaction (kex) of spin-labelled lysozyme and the triplet probes perylene, eosine and anthracene butanoic acid with the haemoproteins were measured in microsomes and in solution by electron paramagnetic resonance and by the registration of delayed annihilation fluorescence. Constants of efficient exchange interactions with the haem groups of myoglobin, haemoglobin, cytochrome c and b5 are 3-22 X 10(7) M-1 s-1 in solution. The experiments with membrane-bound cytochrome P-450 revealed no exchange interactions with the probes located in solution or in the membrane. These results can be accounted for by the deeper incorporation of cytochrome P-450 haem into the protein globule as compared to the other haemoprotein haems studied.
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PMID:Haem localization in haemoproteins by spin and triplet tools. 242 31

A monoclonal antibody (MAb) to a methylcholanthrene (MC)-induced cytochrome P-450, designated MAb 1-7-1, was used for immunohistochemical staining of formalin-fixed tissues from oil- and MC-treated C57BL/6, DBA/2, and [(C57BL/6 X DBA/2) F1 X DBA/2] F2 mice. An avidin-biotin-peroxidase complex immunohistochemical technique was used. For controls, the tissues were also exposed to MAbs 1-48-5 and HyHel-9 (to egg white lysozyme). In liver, MAb 1-7-1 specifically stained the cytoplasm of centrilobular hepatocytes of C57BL/6 mice treated with MC (80 mg/kg) 48 h before kill; staining was not observed with vehicle-treated C57BL/6 mice, with oil- or MC-treated DBA/2 mice, or with comparable antibody concentrations of control MAbs 1-48-5 or HyHel-9. In the F2 mice, about 50% were expected to be MC inducible (AhbAhd). Inducibility phenotype was determined by measuring the conversion of [14C]MC to oxidized and conjugated products by liver homogenates. In freshly fixed material from MC-treated mice, those livers shown by the determination of phenotype to be inducible also stained with MAb 1-7-1, whereas those not induced were immunohistochemically negative. Furthermore, there was a significant positive correlation between degree of staining and the level of MC-metabolizing activity measured biochemically. The immunohistochemical procedure was also accurate in determination of inducibility phenotype of livers that had been in paraffin blocks for up to 2 yr if more concentrated antibody was used. In lung, MAb 1-7-1 stained specifically the alveolar walls and endothelium of blood vessels in MC-induced C57BL/6 mice only; the control MAbs and other mice gave negative results. Similarly, in kidney MAb 1-7-1 stained only glomeruli and interstitial tissue of MC-induced C57BL/6 mice and only endothelium of blood vessels in the colons of these mice. These observations are consistent with induction of the cytochrome P-450 recognized by MAb 1-7-1 in the endothelial cells of extrahepatic tissue. Immunohistochemical staining with MAb thus shows great promise for highly specific localization of particular species of cytochromes P-450 in tissues, for in situ quantification of these enzymes, and for determination of inducibility phenotype with fixed material.
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PMID:Immunohistochemical determination of inducibility phenotype with a monoclonal antibody to a methylcholanthrene-inducible isozyme of cytochrome P-450. 366 9

The authors studied the effect of a long-term intragastric administration to CBA X C57Bl/6 male mice of T-2 toxin in doses of 0.067 mg/kg bw a day (1/100 of the LD50) or 0.33 and 0.45 mg/kg a day (1/20 and 1/15 of the LD50) on the liver content of protein, cytochrome P-450 SH-glutathione and on the activity of 10 lysosomal and microsomal enzymes and glutathione transferase. A dose-dependent increase in the activity of lysosomal hydrolases and glutathione transferase localized in cytosol was revealed together with a fall in the activity of microsomal aniline hydroxylase, carboxyl esterase and epoxide hydrolase. Emphasis is laid on a dose-dependent reduction in the liver of nonsedimented activity of lysosomal enzymes. In T-2 mycotoxicosis, the most sensitive and the most stable parameter was the activity of lysosomal enzymes in blood serum. That activity also declined during the recovery period, namely 3 months after discontinuance of toxin administration. Both groups of mice showed a progressive decrease in the blood leukocyte count and lysozyme content, whereas in the spleen, there was a decrease in the number of antibody-forming cells. It is concluded that biochemical, hematological and hematological characteristics should be taken into consideration in evaluating the chronic action of T-2 toxin.
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PMID:[Biochemical, hematological and immunological criteria for assessing chronic T-2 mycotoxicosis in mice]. 406 Jun 87

Primary structure was determined for the recently cloned f1/BglII-fragment [19] containing 2102 b.p. of the human tissue plasminogen activator (tPA) gene 3' end and adjacent DNA region. Computer analysis has revealed an Alu-repeat 820 b.p. downstream the tPA gene; the sequence proved to have a considerable homology (86-88%) with the Alus from the 3'-untranslated regions (3'UTRs) of cytochrome P-450, lysozyme and p53 protein human mRNAs. The same homology was estimated for this Alu in reversed orientation and Alus from the 3'UTRs of some other human mRNAs. In contrast, the homology between this 3' end tPA gene flanking Alu-repeat and other Alus dispersed throughout the gene introns either direct or reversed, was less than 70%. The polyadenylation signal AATAAA downstream the Alu and two nearby signals CACAG and GTGTT resembling consensus sequences CACAG and YGTGTTYY, respectively, were also detected. The two latter motifs located close to the 3' ends in most mammalian genes are likely to regulate mature mRNA formation. The comparison of the sequenced spaser flank adjacent to the tPA gene with short homologous sequence from the same genomic region primary structure reported previously has revealed discrepancies (substitutions, deletions or insertions) in 21 nucleotide positions. The nucleotide sequence of E. coli uvrB gene fragment (980 b.p.) is also reported. This E. coli gene fragment was cloned accidentally within the f1/BglII-fragment being an artifact of the host-vector system used.
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PMID:[Determination and analysis of the primary structure of a genomic sequence adjacent to the 3'-end of the human tissue plasminogen activator gene]. 778 34