Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.17 (
lysozyme
)
21,489
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The influences of the hydrophilic chain length, morphology and chemical nature have been probed with regard to the adsorption of model proteins onto the surface of soft nanoparticles (crew-cut micelles and polymersomes). The investigations were based on assemblies manufactured from
PEO
m
-b-PLA
n
(poly(ethylene oxide)-b-poly(lactic acid)), which is a well-established block copolymer platform towards the manufacturing of drug delivery vehicles, and PHPMA
m
-b-PDPA
n
(poly([N-(2-hydroxypropyl)]methacrylamide)-b-poly[2-(diisopropylamino)ethyl methacrylate]), which is pH-responsive and therefore potentially able to target damaged cells in slightly acid microenvironments. Besides, protein adsorption onto PHPMA-stabilized nanoparticles has been seldom explored up-to-date. The morphologies were produced using two different approaches (nanoprecipitation and thin-film hydration) and afterwards, the protein-repelling property of the assemblies in model protein environments (BSA - bovine serum albumin,
lysozyme
and IgG - immunoglobulin G) was evaluated. We report that, regardless the morphology, PHPMA
35
-b-PDPA
42
block copolymer assemblies are highly stable with negligible protein binding. On the other hand,
PEO
m
-b-PLA
n
nanostructures are susceptible to protein adsorption and the phenomenon is protein-dependent. The nanoparticles are more susceptible to adsorption of the model positively charged biomacromolecule (
lysozyme
). The adsorption phenomenon is thermodynamically complex with simultaneous endothermic and exothermic processes involved. Although the experimental data highlight that qualitatively the morphology plays negligible effects on the event, fluorescence spectroscopy measurements evidenced that the binding is stronger onto the surface of nanoparticles stabilized by shorter hydrophilic shells. Nevertheless, the adsorption does not affect the secondary structure of the model proteins as confirmed by circular dichroism spectroscopy. Overall, by comparing soft nanoparticles stabilized by
PEO
and PHPMA, the latter is herein proved to be a better choice towards the manufacturing of non-fouling structures (either core-shell or hollow spheres) where even a reasonably short hydrophilic chain confers outstanding protein-repelling feature.
...
PMID:Outstanding protein-repellent feature of soft nanoparticles based on poly(N-(2-hydroxypropyl) methacrylamide) outer shells. 3233 Jul 52
The co-assembly of peptides and proteins in poly(styrene-
block
-ethylene oxide) (PS-
b
-
PEO
) thin films has proven to be a promising method to fabricate polymer-biomolecule functional materials. Contrary to the covalent immobilization of biomolecules on surfaces, co-assembly presents the opportunity to arrange cargo within thin films, which can be released upon exposure to an aqueous environment. The use of a mixed solvent system ensures the solubilization of hydrophobic polymer as well as the solubilization and protection of the biomolecule cargo. However, to produce largely defect-free films of PS-
b
-
PEO
from a solvent mixture containing water is challenging due to the narrow range of solvent miscibility and polymer/protein solubility. This work explores the limits of using a benzene/methanol/water solvent mixture for the production of thin PS-
b
-
PEO
films and provides a template for the fabrication optimization of block copolymer thin films in different complex solvent systems. The film quality is analyzed using optical microscopy and atomic force microscopy and correlated to the solvent composition. By adjusting the solvent composition to 80/18.8/1.2 vol % benzene/methanol/water, it was possible to reliably fabricate thin films with less than 1% macroscopic defect surface coverage. Using the optimized solvent composition, we also demonstrate the fabrication of ordered PS-
b
-
PEO
films containing
lysozyme
. Furthermore, we show the release of
lysozyme
into aqueous media, which highlights the potential use of such films for drug delivery applications.
...
PMID:Polystyrene-
block
-poly(ethylene oxide) Thin Films Fabricated from a Solvent Mixture for the Co-Assembly of Polymers and Proteins. 3311 Sep 64
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