Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

---

Query: EC:3.2.1.17 (lysozyme)
21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The purpose of this immunohistochemical study was to know the degree and direction of differentiation, the mechanism of mesenchymal appearance, and histogenesis in pleomorphic adenomas which were obtained from surgical removed specimens. All specimens were fixed in 10% neutral phosphoate buffered formalin, and embedded in paraffin. Sections were steined with the avidin-biotin peroxidase complex method for keratin, vimentin, desmin and S-100 protein, the peroxidase-antiperoxidase method for lysozyme, and the direct immunoperoxidase method for IgA and secretory component. Normal salivary glands were employed as controls. 1. Solid portion of pleomorphic adenomas were consisted of the focal hypline cells and scattered the duct cell-like cells. The hyaline cells were positive for vimentin and S-100 protein, and the duct cell-like cells were positive for keratin. But one case was found the bundles of spindle-shaped cells which was similar to myoepithelial cells were positive for actin and another case was consisted of the focal duct cell-like cells were observed. 2. In the tubular portion, the duct-like structures were classified into three types. One type was mono-layered structure, another type was double-layered structure and the other type is duct-like structure in the solid portion. Any inner layer of the duct-like structure were positive for keratin. 3. Double-layered duct-like structures were most common type. Among the double-layered structures, hyaline cells mostly made up the outer layered structures. At times, the spindleshaped cells of the outer layer were found. This structures were similar to intercalated portion of normal salivary glands. In one case, inner and outer layer was consisted of keratin positive cells, but outer layer was steined for keratin stronger than inner. This structure was similar to excretory duct of normal salivary glands. 4. IgA and sc were seen at inner cells and in luminal accumulation of the duct-like structures. 5. In the myxoid regions, stellate cells or spindle cells were positive for vimentin, S-100 protein and partial positive for desmin. In the chondroid regions, chondrocyte-like cells were positive for vimentin and S-100 protein. These results seemed to suggest that pleomorphic adenomas were composed of duct cell-like cells and hyaline cells. Duct cell-like cells consisted of the duct-like structure in order to transport secretion which was similar to the duct of normal salivary glands.(ABSTRACT TRUNCATED AT 250 WORDS)
...
PMID:[Immunohistochemical study in pleomorphic adenomas of human parotid gland--with special reference to cytoskeletal filamentous proteins]. 248

In this study immuno-electron microscopy was used to assay, semi-quantitatively, the granule contents of elastase, lactoferrin, lysozyme and myeloperoxidase in human peripheral blood neutrophils from 13 chronic myeloid leukaemia patients in the chronic phase of the disease and from normal non-smoking donors. The fixation conditions that adequately preserved the antibody binding capacities of these antigens and reasonably preserved the ultrastructure of the neutrophils were selected by light-microscopic immunoperoxidase cytochemistry on cytospin smears. Immunogold cytochemistry on LR White resin sections localised elastase and myeloperoxidase to the primary granules, lactoferrin to the secondary granules and lysozyme to both types of granule. When applicable, peroxidase cytochemistry was combined with immunogold staining making it easier to distinguish the primary from the secondary granules. A comparison of the immunolabelling density values obtained for the leukaemic and normal states revealed no significant abnormalities in the immunoreactivity patterns for any of these neutrophil granule antigens in the leukaemic patients. All 13 patients gave normal immunostaining reactivities for these neutrophil granule proteins. Consequently the distribution patterns of these proteins, as shown in this study, cannot be used as indices in distinguishing chronic myeloid leukaemic neutrophils from normal neutrophils.
...
PMID:Analysis of human neutrophil granule protein composition in chronic myeloid leukaemia by immuno-electron microscopy. 255 61

An autopsy case of an 18-year-old Japanese girl with a malignant histiocytosis-like disorder that developed during the course of a hypersensitive reaction to mosquito bite is reported. Episodes of hypersensitive reactions to mosquito bite had been repeated since she was 12 years old and at the age of 18 years she died of acute respiratory failure only 11 days after a mosquito bite. On autopsy, the dermal reaction to the last mosquito bite had already calmed down, and 'poorly differentiated histiocytes', which were presumed from their histochemical characteristics, had remarkably infiltrated into multiple organs, appearing like leukemic infiltration. Only a small number of them were noted in the lymph nodes and the bone marrow. The results of histochemical examination of 'poorly differentiated histiocytes' was as follows: (1) Neither markers for granulocytes (peroxidase and naphthyl AS-D Cl esterase) nor markers for lymphocytes and plasma cells (leukocyte common antigen and immunoglobulins) were detected. (2) Some markers for histiocytes (peanut lectin agglutinin and lysozyme) were positive in some of the proliferated cells. (3) A marker for T-zone histiocyte (s-100 protein) was negative. These results suggested that the proliferated cells included cells of the monocyte-macrophage system. These cells morphologically showed no phagocytic activity and were suggested to be immature histiocytes. Jurco et al. reported (poorly differentiated) malignant histiocytosis consisting of immature histiocytes without phagocytic activity, by using histochemical methods.(ABSTRACT TRUNCATED AT 250 WORDS)
...
PMID:[An autopsy case of malignant histiocytosis-like disorder following hypersensitive reaction to mosquito bite]. 256 94

The interaction of lactoperoxidase with lysozyme and ribonuclease as well as immunoglobulins from cow milk has been investigated. As gel filtration and enzyme kinetics experiments have shown, the lactoperoxidase was slightly activated by complexing to lysozyme, while IgA and IgM were inhibitory for the peroxidase. Oh the other hand, IgG and ribonuclease had no effect on the enzyme activity although the latter did form a complex with the lactoperoxidase. The interaction between the lysozyme and lactoperoxidase appears to be rather specific since the alteration of the lactoperoxidase sugar moiety by periodate oxidation, prevented the formation of the lactoperoxidase-lysozyme complex.
...
PMID:Interaction of lactoperoxidase with enzymes and immunoglobulins in bovine milk. 263 59

The numbers of Langerhans cells and lysozyme-positive macrophages were assessed quantitatively in normal ectocervical epithelium, cervical intraepithelial neoplasia (CIN), microinvasive squamous cell carcinoma (MICA), clinical invasive squamous cell carcinoma (CICA), and koilocytotic atypia using the avidin-biotin-peroxidase complex (ABC) method. The distribution of Langerhans cells was different from that of lysozyme-positive macrophages in that the former were intermingled with the cervical lesion, while the latter were present mainly surrounding the cervical lesion and/or on the edge of the cervical lesion. The numbers of Langerhans cells and lysozyme-positive macrophages in CIN were significantly larger than those in normal ectocervical epithelium and significantly smaller than those in invasive squamous cell carcinoma (MICA + CICA). Langerhans cell number significantly increased as the grade of CIN advanced. In contrast, the number of lysozyme-positive macrophages did not differ significantly between progressive grades of CIN. As for koilocytotic atypia, the numbers of Langerhans cells and lysozyme-positive macrophages in koilocytotic atypia were significantly greater than those in normal ectocervical epithelium but did not differ significantly from those in CIN 1 and CIN 2. With respect to stromal lymphoid infiltration, invasive squamous cell carcinoma with moderate or dense stromal lymphoid infiltration showed significantly greater numbers of Langerhans cells and lysozyme-positive macrophages than that with no or scattered stromal lymphoid infiltration, but such a correlation was not found in CIN.
...
PMID:Immunohistochemical demonstration of histiocytes in normal ectocervical epithelium and epithelial lesions of the uterine cervix. 265 25

Current knowledge about the etiology and histogenesis of keratoacanthoma (KA) is first reviewed, after which the various clinical and histological patterns of this tumor are presented. The differential diagnosis, particularly against spinocellular carcinoma (SCC), and possible forms of therapy are discussed. A series of 90 KAs seen in the period 1976-1986 were investigated histopathologically with reference to the overall architecture, the epithelial differentiation, the behavior towards the adjacent tissue, and the extent and composition of the cellular infiltrate. In addition to routine staining with hematoxylin-eosin and Giemsa, several files have been subjected to immunohistological analysis by means of the peroxidase-antiperoxidase method (PAP) (keratin, lysozyme, epithelial membrane antigen, tissue polypeptide antigen). In 79 cases (88%) the overall architecture found was that of an endo-exophytic, dome-shaped structure with a central crater filled with a plug and with epithelial "lips" at the margins. In 3 KA (3%) a more endophytic picture was seen though no ulceration was observed. In 2 tumors in the very early stages (2%), there was no formation of horn crater and no epithelial lips. Similarly, 5 flat, plaque-like KA (6%) also showed no horn crater. With the exception of 3 aggregated (and/or multicentric) KA (3%), no solitary or multiple types were found. In 88 cases (98%), proliferation of the epidermis was observed mainly in the lateral and basal parts. In 86 KA (96%) there was a relatively well-demarcated, sharp border to the basal tumor portions. In 4 cases (4%) a less precise definition of the outer tumor margins was observed, because of a considerable pseudocarcinomatous, though not truly infiltrative, growth pattern. In 24 KA (27%) there was a strong tendency to formation of one or more sequesters. In the majority of cases, maturation of the epithelial cells was normal. Abnormal multipolar mitoses (7 cases; 8%) and relatively pronounced cellular atypies (8 cases; 9%) were occasionally observed. There was a clear correlation between the composition and extent of the inflammatory infiltrate and the tumor growth stage. In 78 KA (87%) large numbers of intraepithelial neutrophilic microabscesses were shown. In only 13 cases (14%) were substantial numbers of eosinophilic leukocytes observed, and 3 of these tumors (3%) later developed eosinophilic microabscesses.(ABSTRACT TRUNCATED AT 400 WORDS)
...
PMID:[Keratoacanthoma and its clinical variants. Review of the literature and histopathologic analysis of 90 cases]. 265 49

The peroxidase-immunoperoxidase immunocytochemical method was used on 27 saphenous vein coronary artery bypass grafts, which had been resected because of recurrent angina, to identify in situ cellular and humoral elements possibly associated with graft occlusion. Immunostaining was performed on paraffin wax embedded control saphenous vein and graft sections incubated directly with primary antibodies against von Willebrand antigen (vWFAg), fibronectin, fibrinogen, leucocyte common antigen (LCA), lysozyme, vimentin, desmin, platelet factor 4, and thrombospondin. Antigens were visualised by a chromogen providing an orange-red immunoprecipitate at the site of epitope localisation. The intraluminal, amorphous exudate present in most grafts was not composed simply of fibrin or fibrinogen, as previously thought, but was a multiprotein complex including wWFAg, fibronectin, thrombospondin and platelet factor 4. Along with macrophages, these components probably enter the graft after haemodynamic, physical, and chemical injury to, and disruption of, the endothelial cell. Progressive myointimal proliferation and fibrosis of these grafts may be local repetitive responses to macrophages and platelets, cells previously known to participate in vascular disease.
...
PMID:Immunocytochemical features of obstructed saphenous vein coronary artery bypass grafts. 265 29

Nitrosation and acetylation, two histochemical blocking procedures for amino groups, were used to establish the extent to which these groups intervene in the antigen-antibody reaction in immunohistochemistry. We used the peroxidase-antiperoxidase method (PAP) to demonstrate lysozyme in Paneth cells and in lamina propria mononucleocytes of human small intestine as a model system. We studied the relationship of these groups to fixation, concentration of the primary antiserum, and length of blockade, as well as the possibility of reversing blockade as proof of specificity. Our findings support the contention that amino groups are also an important factor in antigen-antibody binding, even in fixed tissue. Fixatives influence the binding process in many ways, with acetylation producing a more successful result than nitrosation in tissue fixed in Bouin without acetic acid, whereas the reverse is true in formaldehyde-fixed tissue.
...
PMID:Histochemical blockade of the antigen-antibody reaction using immunoperoxidase demonstration of lysozyme in paneth cells and lamina propria mononucleocytes of human small intestine as a model system. 265 61

Investigation of vitality was done in paraffin-embedded tissue sections of open skin wounds by demonstration of a neutrophilic reaction marking the cells by naphthol AS-D chloroacetate esterase, by demonstration of enzyme activity by application of lysozyme antibodies and by demonstration of concentration of proteinase inhibitors by application of inhibitor antibodies using the indirect immunohistochemical peroxidase method. Survival time of the wounds could be determined in 10 cases (15-165 mins) and was unknown in 9 other cases of sudden death due to injury of the major vessels or heart. Postmortally inflicted injuries and cases of sudden death due to massive blunt trauma served as controls. In vital injuries, accumulation of proteinase inhibitors, particularly alpha-1-antichymotrypsin and alpha-2-macroglobulin, were demonstrable in the corium parallel to the wound surface. Protein accumulation was observed only sporadically in cases of sudden death and never in cases with postmortally inflicted wounds. Granulocytes and lysozyme were first observed in the corium after a survival time of more than 60 minutes.
...
PMID:[Immunohistochemical assessment of survival from open skin wounds using paraffin sections]. 268 50

The hybridoma, 62H3, which secretes a monoclonal IgG2b with anti-HLA-DR specificity, was expanded in pristane-primed BALB/c mice and the antibody was isolated from the ascitic fluid by affinity chromatography on Protein A-Sepharose. The purified IgG2b antibody was tested by an enzyme immunoassay for antibody activity against a panel of 40 self and non-self antigens. It was found to react strongly with beta-galactosidase, actin, glutamate dehydrogenase, rabbit and human IgG and di- and trinitrophenyl groups; and moderately with tubulin, insulin and phosphorylcholine; but it did not react with various other self and non-self antigens, such as DNA, albumin, keyhole limpet hemocyanin, hen lysozyme and horseradish peroxidase. Fab and Fc fragments were prepared from this IgG2b by papain proteolysis. The Fab fragment possessed the same spectrum of polyreactivities as the native IgG2b, whereas no activity was detected with the Fc fraction. In order to investigate the properties of the antigen binding site, the actin, TNP and rabbit IgG antibody activities were studied in more detail by enzyme immunoassay, Western blot and immunocytochemistry. The monomolecular nature of this multireactivity was confirmed by immunoabsorption analysis. Furthermore, 62H3 monoclonality was also verified by comparative isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis with other monospecific antibodies. The dissociation constants (Kd) of antigen-antibody equilibria in solution were measured. The Kd for actin was 1.11 +/- 0.24 x 10(-5) M and the Kd for TNP-BSA was 8.7 +/- 0.51 x 10(-7) M. No interaction with rabbit IgG could be detected in solution. These findings raise the question of the possible implication in autoimmune pathology or in normal physiology of IgG class polyspecific antibodies with solid-phase restricted cross-reactive rheumatoid factor activity.
...
PMID:Immunochemical studies of a murine polyreactive IgG2b autoantibody with rheumatoid factor activity. 277 Jul 48


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>

---