Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Polyurethane (PU) with zwitterionic side chains has been prepared to resist nonspecific adsorption of proteins. First, dihydroxy-terminated poly(2-(dimethylamino)ethyl methacrylate) (PDEM(OH)(2)) is synthesized by free radical polymerization with 3-mercapto-1,2-propanediol as the chain transfer agent, which polyadds with diisocyanate to yield a PU with PDEM side chains. Such side chains are zwitterionized by 1,3-propane sultone. Proton nuclear magnetic resonance spectroscopy ((1)H NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) show that the zwitterionic side chains are incorporated into the PU. Thermal analysis demonstrates the thermal stability is greatly affected by the content of the side chains. By use of quartz crystal microbalance with dissipation (QCM-D), we have investigated the adsorption of fibrinogen, bovine serum albumin, and lysozyme on a surface constructed by such a PU. It shows the PU has a controllable protein resistance depending on the content of the zwitterionic side chains.
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PMID:Preparation of polyurethane with zwitterionic side chains and their protein resistance. 2122 76

We have developed a novel approach to introduce zwitterions into polyurethane for the preparation of antibiofouling coating. First, the thiol-ene click reaction between 2-(dimethylamino)ethyl methacrylate (DMAEMA) and 3-mercapto-1,2-propanediol (TPG) is used to synthesize dihydroxy-terminated DMAEMA (DMA(OH)2) under UV catalysis. The product has been proved by gel permeation chromatography (GPC), Fourier transform infrared spectrum (FT-IR), proton nuclear magnetic resonance ((1)H NMR), and high resolution mass spectrometry (HRMS). DMA(OH)2 is then incorporated into polyurethane as side groups by polyaddition with diisocyanate and further reacts with 1,3-propane sultone to obtain the zwitterionic polyurethanes. The presence of sulfobetaine zwitterions side groups has been demonstrated by FT-IR and X-ray photoelectron spectroscopy (XPS). Thermal analysis indicates that the thermal stability is decreased with the increasing content of zwitterionions. The antibiofouling property of polyurethanes has been investigated by the measurement of adsorption of fibrinogen, bovine serum albumin (BSA), and lysozyme on the polyurethanes surface using quartz crystal microbalance with dissipation (QCM-D). The results show that the polyurethane coatings exhibit effective nonspecific protein resistance at higher content of zwitterionic side groups.
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PMID:A novel approach for synthesis of zwitterionic polyurethane coating with protein resistance. 2531 Jan 80