Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spores of Bacillus cereus T treated with trichloroacetic acid (6.1--61.2 mM) were compared with untreated spores, and as the concentration of the chemical increased, the following alterations in spore properties were found: (1) the extent of germination decreased irrespective of the germination medium used; (2) the spores became sensitive to sodium hydroxide (1 N) and hydrochloric acid (0.27 N), but not to lysozyme (200 micrograms/ml); (3) loss of dipicolinate increased on subsequent heating; and (4) the spores became more sensitive to heat. However, trichloroacetic acid-treated spores were still viable and there was no significant change in spore components. The mechanism of action of trichloroacetic acid is discussed.
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PMID:Effect of trichloroacetic acid treatment on certain properties of spores of Bacillus cereus T. 4 Nov 62

The relationship between lysozyme and sodium reabsorption by the kidney tubule was studied in the experimental Fanconi syndrome. Female, anesthetized Sprague-Dawley rats were injected intravenously with maleic acid (an inhibitor of sodium transport) neutralized with sodium hydroxide in doses of either 2 or 8 mmol/kg. Clearance studies were performed immediately afterward, and plasma and urine were analyzed for inulin, pH, sodium, glucose, and lysozyme. Two hours after the maleic acid injection, renal cortical tissue was removed and homogenized. Specific activity of Na-K-ATPase was assayed in the light microsomal fraction. The results showed that both concentrations of maleic acid caused significant increases in urinary volume, glucose excretion, and pH. There were significantly correlated decreases in TNafract and TLyfract. The slope of the regression line (TLyfract = 1.03 TNafract - 5.82; r = 0.92) approximated unity. Renal cortical Na-K-ATPase activity was significantly decreased by 25% in the animals receiving 2 mmol maleic acid and 43% in the animals receiving 8 mmol. The evidence suggests that lysozyme reabsorption in the proximal tubule might be mediated directly or indirectly by active tubular transport of sodium, a process that is related to the Na-K-ATPase transport system.
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PMID:Renal handling of lysozyme in experimental Fanconi syndrome. 14 77

The effect of various polycations on the immune response potentiated with poly I:C was studied. It was found that low molecular weight polycations had no potentiating effect. Polylysine was ineffective whereas protamine was superior to lysozyme, poly-arginine, poly-histidine, DEAE-Dextran and histone. A foot-and-mouth disease trivalent vaccine composed of strains A24 Cruzeiro, O1 Caseros and C2 Resende elicited no immune response in swine when adjuvanted with aluminium hydroxide but was effective when emulsified in oil. In general, the immune response was potentiated ten-fold when the emulsion contained poly I:C. The antibody production was in most cases further potentiated by a factor of ten when the nucleic acid double-strand was complexed with 1 : 10 (w/w) DEAE-Dextran. Protamine was as effective, or perhaps even more, than DEAE-Dextran to this effect. Guinea pigs vaccinated with a water-in-oil emulsion type monovalent C3 vaccine showed an increase in antibody production when the vaccine contained poly I:C or poly I:C complexed with 1 : 10 (w/w) protamine.
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PMID:Potentiation of FMD vaccines with polycationic-nucleic acid complexes. 59 39

Total poly(A)-containing RNA prepared from hen oviduct and centrifuged on an isokinetic sucrose gradient displays four peaks of optical absorbance. These have been identified by translation in vitro as lysozyme, ovomucoid, ovalbumin, and conalbumin mRNAs. Isolation and recentrifugation of the peaks results in partial purification of each mRNA. Molecular weights have been determined for the mRNAs on agarose gels containing 20 mM methylmercury hydroxide. Each mRNA possesses a number of apparently untranslated nucleotides ranging from approximately 900 bases for ovalbumin and conalbumin mRNAs to 200 bases for ovomucoid and lysozyme mRNAs. The mRNAs have been copied with avian myeloblastosis virus reverse transcriptase. Each mRNA with the exception of conalbumin gives rise to a high proportion of full length cDNA. Several parameters previously reported to influence the size distribution of cDNA had no effect on the length of cDNA made from any mRNA fraction. The proportion of full length copy does depend on the reverse transcriptase lot.
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PMID:Synthesis of full length cDNAs from four partially purified oviduct mRNAs. 63 80

Spores of Bacillus subtilis NCTC 8236 were exposed to 2% alkaline glutaraldehyde and subsequently subjected to various treatments in an attempt to revive injured spores. Treatment with alkali (sodium or potassium hydroxide or, to a lesser extent, sodium bicarbonate) proved to be most successful. Some revival was achieved after thermal treatment. No revival was obtained with lysozyme or with various types of coat-removing agents. Experiments designed to distinguish between germination and outgrowth in the revival process established that sodium hydroxide (optimum concentration, 20 mmol/l) added to glutaraldehyde-treated spores increased the potential for germination. In contrast, spores which had been allowed to germinate before exposure to low concentrations of glutaraldehyde and then to sodium hydroxide were inhibited at the outgrowth phase to a much greater extent than germinated spores treated with the dialdehyde without subsequent alkali exposure. The results overall are discussed in terms of the possible mechanism and site of action of glutaraldehyde and the practical implications and significance of its use as a sporicide.
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PMID:Possible mechanisms for the revival of glutaraldehyde-treated spores of Bacillus subtilis NCTC 8236. 250 64

Two new laboratory diagnostic tests for chronic glomerulonephritis and pyelonephritis have been developed. The first one is based on electrophoretic mobility of urinary lysozyme under certain conditions, such as the use of 12% polyacrylamide gel with pH of 4.3 and acid electrode buffer with pH of 4.0. After electrophoresis was discontinued, lysozyme position was determined by lysis of Micrococcus lysodeikticus, used as test agents and added to the gel as a suspension prior to polymerization. Urinary lysozyme was found to be in the anode area of the gel in 95% of patients with chronic pyelonephritis, and in its cathode area in 92% of patients with chronic glomerulonephritis. There was no lysozyme in the urine of normal subjects. The other laboratory technique, the ethanol test, is based on comparative assessment of the degree of urinary opacification after ethanol is added in conditions of neutral reaction (following the addition of physiologic saline) and marked alkaline reaction (following the addition of sodium hydroxide solution). The ratio of optic density of the alkaline specimen to that of the neutral specimen was above 1 in patients with chronic glomerulonephritis, and below 1 in patients with chronic pyelonephritis and normal subjects. Examination of biochemical mechanisms of the proposed tests has demonstrated that the pattern of proteinuria is the most important factor affecting the results.
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PMID:[New methods in the laboratory diagnosis of chronic glomerulonephritis and chronic pyelonephritis]. 271 91

Procedures for lyzing staphylococcal cells with the use of ultrasound, lysozyme and a lytic enzyme complex of Actinomyces recifensis var. lyticus, 2435 were compared. The lysis level was estimated by two parameters: lower optical density and protein yield percentage. It was found that ultrasound provided rather high levels of cell destruction reaching 60-68 per cent. The use of lysozyme enabled to destroy 16 per cent of the cells. The enzyme complex of strain 2435 showed high lytic activity with respect to the tested culture. For destroying dense staphylococcal suspensions it appeared necessary to study the effect of preliminary treatment of the cells with various chemical substances on their liability to the effect of the enzyme complex. It was demonstrated that treatment of the cells with 0.01-0.1 M cystein HCl solutions, 0.01-0.02 M sodium dodecylsulfate solutions or 0.05-0.5 M sodium hydroxide solutions increased 2.6-4.7-fold the cell liability to enzymatic hydrolysis. The studies enabled to develop conditions providing complete lysis of 10-percent staphylococcal cell suspension within 5 to 15 minutes under the effect of the lytic enzyme complex of strain 2435. A procedure for isolating cell walls was developed.
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PMID:[Enzymatic lysis of staphylococcal cells and isolation of cell walls]. 337

An in vitro assay for assessing degradative action of neutrophils on basement membrane has been developed. Basement membrane adhering to the base of microtitre wells can be reacted with neutrophils allowing monitoring of the release of cell components or of isotope from labelled membranes. Using either sheep or rat renal membranes labelled with [3H]propionate it was shown that either rabbit anti-sheep basement membrane antibody or rabbit anti-rat nephrotoxic serum promoted degradation of the membranes by neutrophils from rabbits. Studies with proteinase inhibitors showed that the degradation was a result of proteolytic action. Comparisons of the effects of serum or plasma with the effects of heat-inactivated serum or plasma showed that complement did not increase degradative activity but did increase the exocytosis of lysozyme by the cells. Hydroxyl radical was the principal oxygen-derived metabolite produced by the cells but free radical scavengers had no effect on the extent of degradation effected by the cells.
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PMID:Degradation of rat or sheep glomerular basement membrane by rabbit neutrophils in vitro. The roles of proteinases, complement and oxygen-derived radicals. 639 84

The effect of ionic strength and ethylene glycol on the adsorption of bovine serum albumin (BSA) or lysozyme by a commercial aluminium hydroxide or aluminium phosphate adjuvant was studied at pH 7.4 and 25 degrees C. The adsorption of BSA by aluminium hydroxide adjuvant and lysozyme by aluminium phosphate adjuvant was found to be inversely related to ionic strength. This indicates that electrostatic attractive forces contribute to adsorption. The adsorption of lysozyme by aluminium phosphate adjuvant was reduced by the addition of ethylene glycol. However, no change in the adsorption of BSA by aluminium hydroxide adjuvant was noted when up to 40% ethylene glycol was present. This behaviour indicates that hydrophobic forces contribute to the adsorption of lysozyme but not of BSA. However, virtually no adsorption was observed when the protein and the adjuvant had the same surface charge. Thus, attractive forces may not be sufficient to produce adsorption of an antigen by an aluminium-containing adjuvant if electrostatic repulsive forces are present.
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PMID:Contribution of electrostatic and hydrophobic interactions to the adsorption of proteins by aluminium-containing adjuvants. 776 76

The effect of adsorbing two model proteins, bovine serum albumin and lysozyme, on the point of zero charge of aluminium-containing vaccine adjuvants was studied. At physiological pH, the adsorption of the negatively charged albumin (isoelectric point = 5.0) by aluminium hydroxide adjuvant (point of zero charge = 11.1) resulted in a decrease in the point of zero charge. In contrast, the adsorption of positively charged lysozyme (isoelectric point = 9.6) by the negatively charged aluminium phosphate adjuvant (point of zero charge = 5.0) resulted in an increase in the point of zero charge. The surface charge characteristics of the aluminium-containing adjuvant dominated at low protein coverage. In contrast, the surface charge characteristics of the adsorbed protein dominated at high protein coverage. Therefore, the physicochemical properties of the antigen-adjuvant complex and not the adjuvant alone should be considered during vaccine preparation.
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PMID:Effect of protein adsorption on the surface charge characteristics of aluminium-containing adjuvants. 802 56


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