Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.17 (
lysozyme
)
21,489
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A group of chitin-binding proteins were isolated from tuberous roots of Raphanus sativus by affinity chromatography with deaminated regenerated chitin (Fig. 1). SDS-PAGE showed that there are at least five proteins in the sample (Fig. 2-b). Through carboxyl methyl-cellulose chromatography, two chitin-binding proteins with
lysozyme
activity, named as
CBP1
and CBP2 (Fig. 3), were purified to homogeneity with the molecular weights of 26.9 kD and 24.8 kD respectively (Fig. 2-d, e).
CBP1
and CBP2 were found to be bifunctional enzymes with activities of
lysozyme
and chitinase (Figs. 4, 5), but without chitosanase activity (Table 1). The
CBP1
and CBP2 could be specifically absorbed by various forms of chitin, such as powdered, regenerated and colloidal forms chitin (Fig. 6). No disulfide bridge was observed in
CBP1
and CBP2 by reduced/nonreduced one-dimensional SDS-PAGE (Fig. 7).
...
PMID:[Purification and characterization of two chitin-binding proteins with lysozyme activity from roots of Raphanus sativus]. 1695 96
