Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Anacystis nidulans 6301 has been transformed in the light to ampicillin resistance with the plasmid pBR322. Permeaplasts prepared by 2-hr treatment of cells with lysozyme and EDTA are transformed with a 50-fold higher efficiency than that observed for cells. beta-Lactamase is present in A. nidulans transformed either with pBR322 or the plasmid pCH1 as evidenced by hydrolysis of the beta-lactam ring of Nitrocefin in extracts of transformants. beta-Lactamase also can be immunoprecipitated from extracts of [35S]methionine-labeled pBR322 transformants and coprecipitates with ribulose-bisphosphate carboxylase. Expression of the carboxylase is apparently amplified in pBR322 transformants as is that for several soluble proteins in pCH1 transformants. Chromosomal DNA per cell is increased about 6-fold after transformation of A. nidulans 6301 with either pBR322 or pCH1. A 4.3-kilobase-pair plasmid can be isolated from pBR322 transformants in addition to the endogenous plasmids pUH24 and pUH25.
 ...
PMID:Transformation of the cyanobacterium Anacystis nidulans 6301 with the Escherichia coli plasmid pBR322. 308 98

The technique of electron microscopy was used to detect the presence of paracrystal hexagonal inclusions in the cells and spheroplasts of the thermophilic hydrogen bacterium Pseudomonas thermophila. The connection of the inclusions with DNA threads can easily be seen on photomicrographs of the spheroplasts. The cells were disintegrated by freezing and thawing and the resultant homogenates were centrifuged in a sucrose density gradient; up to 80% of the activity of ribulose-1,5-diphosphate carboxylase (RDP carboxylase, EC 4.1.1.39) was found in the fraction of particles containing hexagonal paracrystal inclusions (carboxysomes). Their granular content and the tendency to diffuse were seen at a magnification x100,000. The percentage of the insoluble enzyme was higher in cells in the stationary growth phase than in growing cells. Only 25% of the enzyme activity was detected in the particles after the cells treated with lysozyme had been subjected to osmotic shock. A possible role of carboxysomes in cells as a compartment storing RDP carboxylase is discussed.
 ...
PMID:[Carboxysomes of the thermophilic hydrogen bacterium Pseudomonas thermophila]. 708 15