Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Adamantane-modified compounds are known to form stable complexes with beta-cyclodextrins (beta-CD) by host-guest interactions. In this study, the inclusion complex formed between beta-CD cavities and the adamantane group was evaluated for the elaboration of a cation-exchange support. The synthesis of the chromatographic supports involved three steps: (i) a polymer of beta-CD was grafted to diol-modified silica, (ii) a dextran polymer was modified by both adamantane groups and ionizable COOH functions, (iii) the dextran derivative (Ad-Dex-COOH) was bound to the chromatographic support by complexation between the adamantane groups of the dextran and beta-CD cavities of the support. The polymer immobilization on the beta-CD support was successful as the resulting support exhibited weak cation-exchange properties. The stationary phase was easy to prepare under mild conditions (aqueous media, room temperature) and was quite stable when using aqueous mobile phases. The chromatographic behaviour of model proteins was studied in isocratic elution by examining the effect of salt concentration in the buffer on retention. A mixed retention mode was found for lysozyme, revealing both electrostatic and hydrophobic interactions with the stationary phase.
 ...
PMID:Immobilization of a (dextran-adamantane-COOH) polymer onto beta-cyclodextrin-modified silica. 1523 87