Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

2 low-molecular cysteine-proteinase inhibitors were purified from human tonsillar tissues: an acid cysteine proteinase inhibitor (ACPI), and a neutral cysteine proteinase inhibitor (NCPI). Their biochemical and immunological characteristics appeared to be identical to those inhibitors which we have identified in other human tissues in previous studies (epidermis and spleen). An immunohistological analysis revealed in tonsillar squamous epithelium a strong and consistent immunoreactivity for both inhibitors. In the tonsillar lymphatic tissue, ACPI-immunoreactivity appeared to be a characteristic mainly of dendritic reticulum cells whereas a prominent NCPI-immunoreactivity was confined mostly for the histiocytic reticulum cells in lymphoid secondary follicles. We also compared the distribution of the 2 immunoreactive inhibitors with the immunohistology as revealed by antisera raised against keratin and muramidase. Limitations of the immunohistochemistry of cysteine proteinase inhibitors are discussed in the light of an extensive trial of various fixation procedures. We deem that proposal of Barrett (1984) for the nomenclature of cysteine proteinase inhibitors is appropriate. According to it the ACPI is the cystatin A, and the NCPI is the cystatin B.
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PMID:[Low-molecular cysteine protease inhibitors in the human palatal tonsil]. 242 40

The hemocytes of invertebrates play key roles in both cellular and humoral immune reactions by phagocytosis or delivering immune factors such as lectin and anti-microbial peptides. Bacterial infection causes changes in components such as lectins, anti-bacterial peptides, and lysosomal enzymes of plasma or hemolymph in molluscs. Previously, we found that infection with the protozoan parasite, Perkinsus, increases lectin synthesis in hemocytes. In order to investigate the patterns of genes expressed in Manila clams (Ruditapes philippinarum) infected with the protozoan parasite Perkinsus olseni, we constructed a cDNA library and sequenced 1850 clones (expressed sequence tags). A total of 79 ESTs, were related to 29 functional immune genes such as C-type lectin, lysozyme, and cystatin B, in Manila clams. Lectins were the largest group of immune-function ESTs found in our Manila clams library. Among 7 lectin clones, two full length cDNAs of lectins were cloned. MCL-3, which is a simple C-type lectin composed of 151 amino acids, has a relatively short signal sequence of 17aa and single carbohydrate-recognition domain (CRD) of approximately 130 residues. It is highly homologous to eel C-type lectin. The sequence of mc-sialic acid-binding lectin consists of 168 amino acid residues with molecular weight of 19.2 and shows high homology to sialic acid-binding lectin from the snail, Cepaea hortensis. The expression of 7 different lectins in hemocytes was analyzed by RT-PCR using gene-specific primers. Hemocytes from Perkinsus-infected clam expressed different sets of lectins than with Vibrio infection. These results demonstrate that several lectins are involved in Manila clam innate immunity and different challenges induce expression of different lectins.
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PMID:Analysis of EST and lectin expressions in hemocytes of Manila clams (Ruditapes philippinarum) (Bivalvia: Mollusca) infected with Perkinsus olseni. 1669 Jan 26

The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC-MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR.
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PMID:Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules. 2160 66