Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In the neonatal suckling mouse, the antibody response to HEL-CFA can be inhibited by administration of certain anti-HEL monoclonal antibodies to the mother. The murine primary response to hen egg-white lysozyme (HEL), which can be elicited in A/J mice as early as 7 days of age, is characterized by a predominant specificity that includes the 3 N-terminal amino acids of HEL (TIP-dependence) and by a predominant idiotype, IdXE. A panel of murine IgG1 anti-HEL mAbs was administered to the suckling offspring via the mother. These mAbs were not equivalent in their effects on the offspring. Only two of six IgG1 mAbs, 2F4/2E5 (IdXE-positive, TIP-dependent) and 2D1 (IdXE-negative, TIP-independent), consistently induced suppression of the response of A/J offspring when immunized at 16-20 days of age with HEL-CFA. Suppression averaged 71% for 2F4/2E5 and 74% for 2D1 and was always statistically significant (P less than .05) when 275 micrograms mAb was administered IP to the mother within 24 hr postpartum. Since 2D1 is IdXE-negative and TIP-independent, neither of these properties appears to be crucial for suppression. Differences in transfer of the mAbs from the mother to the offspring or differences in catabolism of the mAbs in the offspring were not detected. When various characteristics of the mAbs such as affinity, idiotypy, and fine specificity were considered, there was no single factor which determined suppression. One of the two mAbs that suppressed the offspring response, 2D1, is idiotypically highly connected in the anti-HEL mAb panel. This observation suggests that idiotypic interactions in the developing neonatal repertoire with subsequent perturbation of T and B cell repertoire development may be an area for future investigation.
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PMID:Inhibition of offspring response to HEL-CFA by administration of anti-HEL MAB to the mother is not related to the predominant idiotype, IdXE, or specificity of the MAB. 170 Jul 39

The adult primary murine plaque-forming cell (PFC) response to hen egg-white lysozyme (HEL) is characterized by a predominant idiotype (IdXE) (congruent to 50% of population) and a predominant specificity (congruent to 50% of population) for a determinant dependent on the 3 amino-terminal residues of HEL (TIP-dependent PFC). IdXE and TIP specificity, however, are not congruent: approximately 1/3 of each population do not overlap (1). To determine whether these characteristics result from a prolonged selection process during development, we compared the neonatal A/J response profile to HEL-CFA with the adult A/J response and found that the adult pattern was present for mice immunized as early as 5 days of age. At 5 days, A/J splenic PFC were 80% TIP dependent, 71% IdXE positive compared to the adult levels of 56% (+/- 14) TIP dependent, 43% (+/- 19) IdXE positive. To attempt to address the B cells directly and avoid the need for antigen-specific T-dependent processes, we studied the PFC response to HEL-LPS conjugates in adult and 12-day-old A/J mice. At the earliest age studied (12 days), the indirect splenic PFC had similar idiotypy and specificity as the adult, and the response was similar to that induced by HEL-CFA. Although the absolute IgM PFC level was equal in adult and neonate, only 11% of the adult PFC response was IgM while a large proportion of the 12-day-old HEL-LPS response was IgM. This IgM PFC response was also characterized by the same idiotypy and specificity as the IgG PFC response. These results suggest that the characteristic adult mosaic of specificity and idiotypy in the anti-HEL response may exist prior to antigenic stimulation since it occurs as early as 5 days after immunization and in the IgM PFC responses. Although IdXE predominance may merely reflect the germ line repertoire, T cells may also be involved in an idiotype-based selection, the mechanism of which has not been determined.
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PMID:Primary response to lysozyme (HEL) and HEL-LPS in neonatal A/J mice: presence of characteristic adult pattern of regulatory idiotype and fine specificity restriction. 264 24