Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.17 (lysozyme)
 21,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Serum lysozyme levels were compared in patients with Crohn's disease (CD) and chronic ulcerative colitis (CUC) to determine if the two diseases could be differentiated by this parameter. The lysozyme concentrations were measured by three different methods: 1. the turbidimetric clearance of Micrococcus lysodeikticus utilizing egg white lysozyme as a standard; 2. the lysoplate assay of Osserman and Lawlor using a human lysozyme standard obtained from Dr. Osserman and 3. the lysoplate assay obtained as a commercial kit using human lysozyme as a standard. A series of 19 CD and 23 CUC patients were compared. Contrary to the report of Falchuk, et al, the serum lysozyme levels by any of the three methods were not statistically significantly different in the two diseases. The serum levels of those with severe, moderate, or inactive disease were also not significant for CD or CUC nor in serially followed patients did levels always correspont to changes in clinical course.
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PMID:Serum lysozyme levels in inflammatory bowel disease. 86 43

The purpose of this study was to develop an effective pathogenetically validated method for the treatment of puerperants with pyoseptic diseases, making use of reflex therapy, and clinical assessment of this method. The study has involved 190 puerperants, in 50 of them the postpartum period ran a normal course and the rest 140 developed pyoseptic complications. Case histories were analyzed, clinical and laboratory tests (blood proteins, Mancini's test for immunoglobulins, Dorofeichuk's blood plasma lysozyme test) were carried out. Vegetative regulation was studied with the use of a vegetative test kit. A method for reflex therapy of the detected disorders of the vegetative regulation associated with puerperal pyoseptic diseases has been developed, resulting in strengthening of the defense potential of the body. Clinical assessment of the method has confirmed its high efficacy.
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PMID:[A combined reflex method in the treatment of suppurative-infectious diseases in the postpartum period]. 162 10

A cDNA fragment of about 860 bp corresponding to the c33-c gene in the non-structural region 3 (NS3) of HCV genome was obtained from one plasma derived from a Chinese HCV carrier who came from Tai' an of Shandong Province, China by the application of reverse transcription (RT) and polymerase chain reaction (PCR) techniques. After the sequence of the cDNA fragment was determined and compared with the equivalent region of the HCV-I (HCV-US) and HCV-II (HCV-BK) genomes, the nucleotide/amino acid sequence homologies were found to be 79.2%/91.3%/ and 91.3%/93.9%, respectively. The prokaryotic expression vector pBV220 was employed for the overproduction of c33-c native recombinant protein in E. coli cells. The expression products were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting with antisera of chronic hepatitis C patients, and a molecular weight 31 kD of c33-c viral protein was shown to account for 14% of the total cellular soluble proteins. This product was extracted from the bacterial lysate by lysozyme, Triton X-100 and urea treatment, and purified through ion exchange chromatography. The purified c33-c protein combined with a branch peptide MAP-C-19 representing immunodominant epitopes on the nucleocapsid region of HCV genome was used to develop a Chinese HCV EIA 2nd-generation diagnostic kit for the detection of anti-HCV antibodies. Its specificity, sensitivity and reproducibility were all in keeping with the indexes of the national standard for the quality control of the HCV diagnostic kit. The agreement rate between our kit and Abbott company's HCV EIA second-generation diagnostic kit was 99.33%, and the identified rate of positive anti-HCV of our kit was 2% more than that of the Abbott company's kit.
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PMID:cDNA cloning of c33-c antigen gene derived from NS3 region of Chinese HCV genome, expression in Escherichia coli and development of HCV EIA second-generation diagnostic kit. 752 Jul 1

A method for the preparation of P1 DNA is presented, which allows the direct sequencing of ends of inserts in genomic P1 clones using the Applied Biosystems 373A DNA Sequencer and the Dye Terminator sequencing methodology. We surveyed several common methods of DNA preparation including alkaline lysis, Triton-lysozyme lysis, CsCl density-gradient purification, and a commercial column matrix DNA purification kit manufactured by Qiagen. We found that a modified alkaline lysis preparation of P1 DNA was most successful for generating P1 DNA that could be sequenced directly. We also noted that the host bacterial strain from which the P1 DNA was purified dramatically affected the quality of sequencing templates. The bacterial strains NS3145 and NS3529, in which the Drosophila melanogaster and human P1 genomic libraries are harbored, routinely yielded poor-quality sequencing templates. However, the bacterial strain DH10B routinely yielded P1 DNA that was sequenced successfully. A bacterial mating scheme is presented that exploits gamma delta transposition events to allow the transfer of P1 clones from the library host strain to DH10B. Using either an SP6 or a T7 primer, an average of 350 base pairs of DNA sequence was obtained with an uncalled base frequency of approximately 2%. About 4% of P1 end sequences generated corresponded to unique Drosophila loci present in the Genbank database. These single-pass DNA sequences were used to design sequence-tagged site markers for physical mapping studies in both humans and Drosophila.
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PMID:Direct sequencing of terminal regions of genomic P1 clones. A general strategy for the design of sequence-tagged site markers. 771 Jul 76

This study evaluated the Promega Magic Minipreps (MM) (Promega Corporation, Madison, WI) DNA purification system for use in plasmid analysis of common nosocomial bacterial pathogens. The MM system is a kit that includes lysis solutions and buffers and incorporates a minicolumn of DNA binding resin for recovery of plasmid DNA. The MM system was used according to the manufacturer's directions to recover plasmids for agarose gel electrophoresis from clinical isolates of Acinetobacter calcoaceticus, Enterobacter cloacae, and Klebsiella pneumoniae. For Salmonella enteritidis and Staphylococcus aureus, lysozyme and lysostaphin, respectively, were used for pretreatment. Plasmid DNA from ten isolates could be recovered in approximately one hour with very little manipulation and no phenol/chloroform extractions and was suitable for restriction endonuclease digestion. Compared with a standard miniprep protocol, the MM system was much easier to perform and resulted in significant cost savings due to a 50% reduction in technologist time. The authors conclude that the MM system is a convenient and cost-effective method for clinical microbiology laboratories for recovering plasmid DNA from nosocomial bacterial pathogens.
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PMID:Evaluation of a commercial DNA purification system for plasmid analysis of nosocomial bacterial pathogens. 837 41

Previous studies have shown that immunohistochemical stains for histiocytes are immunoreactive for melanomas. Accordingly, their value in differentiating histiocytes and histiocytic lesions from melanomas was questioned. PG-M1, the most specific histiocytic marker, was not evaluated in these studies. Our aims were to assess the reactivity of PG-M1 with a series of primary cutaneous and metastatic melanomas and to establish the potential usefulness of this antibody in the differentiation between histiocytes and histiocytic tumors and melanomas. PG-M1 staining was performed in 50 primary cutaneous and metastatic melanomas. For comparison, additional sections were stained with KP-1 and lysozyme (commonly used as histiocytic markers) and with S-100 and HMB-45 (commonly used as melanoma markers). The intensity (1+, 2+) and extent (1+ to 4+) were recorded semiquantitatively. PG-M1 stained weakly (1+) and focally (2+) only four cases of melanoma (8%). In contrast, histiocytes were strongly reactive for PG-M1 in all cases, being readily differentiated from melanoma cells including the positive cases. KP-1 stained melanoma cells in 44 cases (88%), lysozyme in 11 cases (22%), S-100 in 50 cases (100%), and HMB-45 in 48 cases (96%). No changes were found after restaining of selected KP-1 and lysozyme positive melanomas using an endogenous avidin/biotin blocking kit. PG-M1 is helpful in discriminating histiocytes and histiocytic lesions from melanoma cells. We recommend its inclusion in any antibody panel put together to distinguish between them.
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PMID:The histiocytic marker PG-M1 is helpful in differentiating histiocytes and histiocytic tumors from melanomas. 1237 44

The aim of this study was to evaluate real-time PCR (LightCycler) kits for the detection of methicillin-resistant Staphylococcus aureus in enrichment broth using 200 sets of patient screening swab samples. One hundred sets were processed using a lysostaphin/lysozyme extraction method and a further 100 were tested using a column extraction kit. The PCR kits with lysostaphin/lysozyme lysis showed 95.7% sensitivity, 90.8% specificity, and negative and positive predictive values of 98.6% and 75.9%, respectively, compared with 88%, 95.9%, 84.6% and 95.9%, respectively, with the column extraction kit.
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PMID:Rapid detection of methicillin-resistant Staphylococcus aureus from screening enrichment broths by real-time PCR. 1592 61

Hen's egg white protein is a major cause of food allergy, and a considerable number of countries have introduced labeling directions for processed food products. To control compliance with these regulations, analytical assays for the detection of egg in manufactured foods have been developed. In this study, we have tested the performance of 3 commercially available kits for quantitative egg analysis using 6 model heat-processed foods. The 3 assays worked well under standard conditions with soluble egg white proteins, but only the kit using a denaturing-reducing extraction buffer detected egg in complex heat-treated food matrixes. The differently extracted food samples were further used to evaluate the stability and allergenicity of the egg white allergens ovalbumin, ovomucoid, ovotransferrin, and lysozyme with polyclonal anti-egg antibodies and sera of 6 patients with egg allergy. It could be shown that differences in egg protein extractability have a significant impact on the interpretation of study results.
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PMID:Extractability, stability, and allergenicity of egg white proteins in differently heat-processed foods. 1747 13

The purpose of this work is to establish the application of a fully automated microfluidic chip based protein separation assay in tear analysis. It is rapid, requires small sample volumes and is vastly superior to, and more convenient than, comparable conventional gel electrophoresis assays. The protein sizing chip technology was applied to three specific fields of analysis. Firstly tear samples were collected regularly from subjects establishing the baseline effects of tear stimulation, tear state and patient health. Secondly tear samples were taken from lens wearing eyes and thirdly the use of microfluidic technology was assessed as a means to investigate a novel area of tear analysis, which we have termed the 'tear envelope'. Utilising the Agilent 2100 Bioanalyzer in combination with the Protein 200 Plus LabChip kit, these studies investigated tear proteins in the range of 14-200 kDa. Particular attention was paid to the relative concentrations of lysozyme, tear lipocalin, secretory IgA (sIgA), IgG and lactoferrin, together with the overall tear electropherogram 'fingerprint'. Furthermore, whilst lens-tear interaction studies are generally thought of as an investigation into the effects of tears components on the contact lens material, i.e. deposition studies, this report addresses the reverse phenomenon--the effect of the lens, and particularly the newly inserted lens, on the tear fluid composition and dynamics. The use of microfluidic technology provides a significant advance in tear studies and should prove invaluable in tear diagnostics and contact lens performance analysis.
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PMID:Tear analysis and lens-tear interactions. Part I. Protein fingerprinting with microfluidic technology. 1749 10

A series of experiments were performed in an attempt to extract genomic DNA from a small number of Eimerian oocysts. Sonication, ammonia, ethanol and lysozyme were all found to be unsuitable for the digestion of Eimeria oocysts. The chemicals and enzyme given were not capable of either disruption or digestion of oocysts for nucleic acid extraction. They had the capability of penetrating the oocyst wall but could not break-up the oocyst wall. It is impossible to obtain nucleic acid from Eimeria oocysts if the wall is not broken-up. In this study oocyst disruption was achieved using a simple but highly effective treatment regime involving sodium hypochlorite treatment, osmotic shock and proteinase K digestion. Following the disruption of the oocyst walls, a commercially available nucleic acid purification kit (Wizard DNA Purification Kit, Promega) can be used to prepare high quality nucleic acid.
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PMID:A novel procedure for total nucleic acid extraction from small numbers of Eimeria species oocysts. 1791 54


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